Objective To observe the expressions of 14-3-3 ζ and β-catenin in human TSUB>1/SUB> nonsmall cell lung cancer(NSCLC), and to investigate the relationship between the expressions of 14-3-3 ζ and β-catenin and progression and invasion in NSCLC. Methods The expressions of 14-3-3 ζ and β-catenin were detected by immunohistochemistry, double fluorescent, laser scanning confocal microscopy and Western blotting in NSCLC and normal lung. Results Upregulation of 14-3-3 ζ was observed significantly in stage TSUB>1/SUB> NSCLC in contrast to that in normal lung. The increased 14-3-3 ζ protein level was correlated with differentiation degree and lymph node metastasis, but it was no correlation with histological type. Decreased expression of β-catenin was observed in NSCLC in contrast to that in normal lung. The expression of β-catenin was positively related to differentiation degree and was negatively related to the lymph node metastasis, and there was no relationship between β-catenin expression and histological type. Conclusion 14-3-3 ζ a