［Abstract］ Objective To observe the drug resistance at different stages of EM>Candida(C.) albicans /EM>biofilm formation. Methods To form the biofilm in glass slide, then observe the biofilm morphology by inverted microscope at different development stages(2, 4， 8, 24, 48 hours).Using National Committee for Clinical Laboratory Standards(NCCLs) microdilution to detect drug resistance of dissociative EM>C.albicans/EM> and 2,3 bis(2methoxy 4 nitro 5 sulfophenyl) 5(phenylamino) carbonyl 2H tetrazolium hydroxide(XTT) reduction method to test the change of EM>C. albicans/EM> drug resistance in biofilm. Results Antifungal resistance of biofilmgrown cells increased as the biofilm maturation. Compared with dissociative C.albicans strains, the drug resistance was stronger more than 100 times. Conclusion There is positive cor