Objective To generate transgenic mouse embryos by somatic cell nuclear transfer (SCNT), and compare effects of different donor cells on developmental potential of cloned embryos. Methods Mouse embryo fibroblasts and mouse embryonic stem (ES) cells were transfected with a plasmid (pCE-EGFP-IRES-NEO) containing the enhanced green fluorescent protein (GFP) and neomycinresistant (Neor) genes by electroporation. Transgenic cell lines were obtained for SCNT after 14 days selection with G418. At the same time, SCNT was performed using cumulus cells and normal fibroblasts as control. Results There was no significant difference in blastocyst rates between reconstructed embryos derived from transgenic fibroblasts and nontransgenic ones (154/160,19.23% vs 22.91%, EM>P/EM>＞0.05). Reconstructed embryos from transgenic ES cells showed higher blastocyst developmental rates than that from transgenic fibroblasts donor (152/154,41.54% vs 19.23%, P<0.05). Cumulus cloned embryos had better developmental potential than that of fibroblasts cloned ones (171/160, 41.17% vs 22.91%, P<0.05). Conclusion Our results showed that transgenic donor cells did not affect EM>in vitro/EM> developmental potential of mouse SCNT embryos. Using EGFP maker, mouse transgenic blastocysts could be produced effectively