Selection of developmentally competent pig oocytes using brilliant cresyl blue staining

Acta Anatomica Sinica ›› 2009, Vol. 40 ›› Issue (2) : 340-343.

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Acta Anatomica Sinica ›› 2009, Vol. 40 ›› Issue (2) : 340-343. DOI: 10.3969/j.issn.0529-1356.2009.02.035
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Selection of developmentally competent pig oocytes using brilliant cresyl blue staining

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Abstract

P>Objective To enhance efficiency of EM>in vitro/EM> embryos culture system of pig using brilliant cresyl blue (BCB) stain. Methods To stain the cumulusoocyte complexes through BCB then evaluate oocytes maturation rates and developmental competence of early embryos. Results The percentage (91.6%) of the MⅡ stage oocytes of pigs was higher in the BCBcolorable group (BCBSUP>+/SUP> ) than those (64.0%) in the noncolorable (BCBSUP>-/SUP>) and control (77.5%) groups (EM>P/EM><0.05). The pig parthenogenetic blastocyst rate (34.9%) was significantly higher in the BCB+ group than those in the control and BCBSUP>-/SUP> groups, (23% and 9.5%) respectively (EM>P/EM><0.05). The rate (23.9%) of pig somatic cell nucleus-transferred embryos into blastocyst was significantly higher in the BCBSUP>+/SUP> group than those in the control and the BCB

Key words

Oocyte / Nuclear transfer / Brilliant cresyl blue (BCB)staining / Pig

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Selection of developmentally competent pig oocytes using brilliant cresyl blue staining[J]. Acta Anatomica Sinica. 2009, 40(2): 340-343 https://doi.org/10.3969/j.issn.0529-1356.2009.02.035

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