Objective To discriminate the precise positions of the deep cerebellar nuclei for functional imaging research on the cerebellum and stereotactic operation of the correlative motor disorders. Methods After CT and MRI examinations to exclude any organic lesion，one head specimen of Chinese adult male was selected from three cadavers，and embedded with gelatin and frozen under profound hypothermia. Subsequently，the specimen was sliced into 0.1mm transverse serial sections along AC-PC line with SKC500 computerized freezing milling machine at -15℃ in a low temperature laboratory. The serial sections were photographed with a high-resolution digital camera and saved in computer files. Finally，the representative sections were meticulously observed and after matching the sectional levels with the MR scans，the position and morphology of dentate nuclei were compared to the corresponding 3.0 Tesla EM>in vivo/EM> MRI. Results In transverse sections involving with cerebellum, about 620 layers were obtained and among the total，the dentate nucleus，fastigial nucleus，globose nucleus and emboliform nucleus occurred in about 145,12,25 and 20 layers respectively. The nuclei were four in number on either side，the dentate nucleus localized on majority of the thin sections and appeared as a convoluted band of gray matter. The nucleus fastigii was situated to the middle line at the anterior end of the superior vermis，and over the roof of the fourth ventricle. The emboliform nucleus lay immediately to the medial side of the dentate nucleus，and partly covering its hilus. The globose nucleus was an elongated mass，directed antero-posteriorly，and placed medial to the emboliform nucleus. Conclusion The position and morphology of the deep cerebellar nuclei could be observed cle