Cytoplasmic transport of monocarboxylate transporter 1 in oligodendrocyte precursor cells induced by low glucose

doi:10.16098/j.issn.0529-1356.2026.03.005

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Acta Anatomica Sinica ›› 2026, Vol. 57 ›› Issue (3) : 306-312. DOI: 10.16098/j.issn.0529-1356.2026.03.005

Cell and Molecules Biology

Cytoplasmic transport of monocarboxylate transporter 1 in oligodendrocyte precursor cells induced by low glucose

ZHANG You-li¹, ZHOU Peng², ZHANG Xiao-yan^1*

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Abstract

Objective To investigate the mechanism underlying the translocation of monocarboxylate transporter 1 (MCT1) from the cytoplasm to the plasma membrane in oligodendrocyte precursor cells (OPCs) under early low-glucose conditions. Methods OPCs were cultured in low-glucose medium. Intracellular cyclic adenosine monophosphate (cAMP) levels were quantified using a chemiluminescence immunoassay kit. Expression levels of sodum\|potassium adenosine triphosphatase (NaK\|ATPase) and MCT1 were analyzed by Western blotting. Mitochondrial membrane potential was assessed by flow cytometry, and the phosphorylation status of MCT1 was determined by protein mass spectrometry. Immunofluorescent staining was performed to examine the co-localization of MCT1 with clathrin and caveolin-1 under low-glucose conditions or after treatment with the cAMP agonist forskolin. Finally, the effect of sodium pyruvate on MCT1 expression was evaluated. Results Low-glucose conditions triggered a significant stress response in OPCs. Under these conditions, phosphorylation of MCT1 enhanced its interaction with clathrin. Moreover, the translocation of MCT1 was dependent on cAMP signaling, and sodium pyruvate upregulated MCT1 expression. Conclusion Under low-glucose conditions, cAMP and clathrin facilitate the trafficking of phosphorylated MCT1 from the cytoplasm to the plasma membrane.

Key words

/ "> Monocarboxylate transporter 1│ Co-translocation│ Cyclic adenosine monophosphate│ Clathrin│ Oligodendrocyte precursor cell│ Immunoprecipitation

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ZHANG You-li, ZHOU Peng, ZHANG Xiao-yan. Cytoplasmic transport of monocarboxylate transporter 1 in oligodendrocyte precursor cells induced by low glucose[J]. Acta Anatomica Sinica. 2026, 57(3): 306-312 https://doi.org/10.16098/j.issn.0529-1356.2026.03.005

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