Effect of baohuosideⅠon Aβ25-35-induced mitochondrial autophagy and apoptosis in SH-SY5Y cells by PINK1/Parkin signaling pathway

doi:10.16098/j.issn.0529-1356.2026.03.004

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Acta Anatomica Sinica ›› 2026, Vol. 57 ›› Issue (3) : 298-305. DOI: 10.16098/j.issn.0529-1356.2026.03.004

Effect of baohuosideⅠon Aβ_25-35-induced mitochondrial autophagy and apoptosis in SH-SY5Y cells by PINK1/Parkin signaling pathway

BAI Yan-mei¹, YANG Guo-feng², ZHANG Zhen-yuan², GAO Juan¹, LI Jing¹, LI Ken¹, ZHANG Jing¹, CUI Yan-li¹, WANG Sen¹*

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Abstract

Objective To investigate the effect of baohuosideⅠ(BaoⅠ) on β-amyloid protein 25-35（Aβ_25-35）-induced mitochondrial autophagy and apoptosis in SH-SY5Y cells by adjusting the PTEN-induced kinase 1/Parkin (PINK1/Parkin) signaling pathway. Methods SH-SY5Y cells in logarithmic growth phase were separated into normal group, Aβ_25-35 group, low concentration baohuosideⅠ(L-Bao Ⅰ) group, high concentration baohuosideⅠ(H-Bao Ⅰ) group, H-Bao Ⅰ+ negative control（NC）small interfering RNA (siRNA）group, and H-Bao Ⅰ+PINK1 siRNA group. Each group was repeated three times. Cell proliferation and apoptosis were detected by MTT and flow cytometry, respectively. The mRNA expression levels of PINK1 and Parkin were detected by Real-time PCR. The autophagy level and mitochondrial membrane potential were detected by transmission electron microscopy and JC-1. The autophagy-related proteins Beclin 1, microtubule-associated protein 1 light chain 3(LC3)Ⅱ, autophagy substrate protein P62, Bax, rabbit anti-Bcl-2, and rabbit anti-LC3Ⅰ expression were detected by Western blotting. Results The Aβ_25-35 group had lower absorbance value at 570 nm (A₅₇₀), expression of PINK1 mRNA, Parkin mRNA, mitochondrial membrane potential, expression of PINK1, Parkin, autophagy related proteins Beclin-1, Bcl-2 and LC3Ⅱ/LC3Ⅰ than the normal group, and higher apoptosis rate, and expression of P62 and Bax than the normal group (P<0.05). The different dose BaoⅠgroups had higher A₅₇₀, expression of PINK1 mRNA, Parkin mRNA, mitochondrial membrane potential, expression of PINK1, Parkin, Beclin-1, Bcl-2 and LC3Ⅱ/LC3Ⅰthan the Aβ_{25-35} group, and lower apoptosis rate, and expression of P62 and Bax than the Aβ_25-35 group (P<0.05). The H-BaoⅠ+ PINK1 siRNA group had lower A₅₇₀, expression of PINK1 mRNA, Parkin mRNA, mitochondrial membrane potential, expression of PINK1, Parkin, Beclin-1, Bcl-2 and LC3Ⅱ/LC3Ⅰ than the H-Bao Ⅰ+ NC siRNA group, and higher apoptosis rate, and expression of P62 and Bax than the H-Bao Ⅰ+ NC siRNA group (P<0.05). Conclusion Bao Ⅰ can activate mitochondrial autophagy induced by Aβ_25-35 in SH-SY5Y cells, inhibit cell apoptosis, and its mechanism is related to the PINK1/Parkin signaling pathway.

Key words

/ "> BaohuosideⅠ│PTEN-induced kinase 1/Parkin signaling pathway│β-Amyloid protein 25-35│Mitochondrial autophagy│Western blotting│SH-SY5Y cell

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BAI Yan-mei, YANG Guo-feng, ZHANG Zhen-yuan, GAO Juan, LI Jing, LI Ken, ZHANG Jing, CUI Yan-li, WANG Sen.

Effect of baohuosideⅠon Aβ_25-35-induced mitochondrial autophagy and apoptosis in SH-SY5Y cells by PINK1/Parkin signaling pathway

[J]. Acta Anatomica Sinica. 2026, 57(3): 298-305 https://doi.org/10.16098/j.issn.0529-1356.2026.03.004

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