Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes induced by rehmannia glutinosa polysaccharide combined with basic fibroblast growth factor

LIANG  Wei-hong1; 2  YANG  Hui-ying1; 2  XU  Xin-ru1; 2  SUN  Hao-dan1; 2  L ü  Yang1 WANG  Hai-ping1; 2*

doi:10.16098/j.issn.0529-1356.2025.06.007

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Acta Anatomica Sinica ›› 2025, Vol. 56 ›› Issue (6) : 681-687. DOI: 10.16098/j.issn.0529-1356.2025.06.007

Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes induced by rehmannia glutinosa polysaccharide combined with basic fibroblast growth factor

LIANG Wei-hong^1,2 YANG Hui-ying^1,2 XU Xin-ru^1,2 SUN Hao-dan^1,2 LÜ Yang¹ WANG Hai-ping^1,2*

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Abstract

Objective To explore the effects of pretreatment with rehmannia glutinosa polysaccharide (RGP) and basic fibroblast growth factor (FGF-2) alone or in combination on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into cardiomyoid cells and the related mechanisms. Methods BMSCs pretreated with FGF-2 and RGP alone or in combination were cultured for 1, 2, and 4 weeks. The expression levels of NKx2.5 and GATA-4 were detected by Real-time PCR. BMSCs pretreated with FGF-2 or RGP alone and PI3K/Akt signaling pathway inhibitor LY294002 were used to detect the expression levels of myocardial specific proteins and related pathway proteins by Western blotting. Results Compared with the blank control group, pretreatment with FGF-2 or RGP alone increased the expression rate of myocardial specific markers, and the effect of combined pretreatment with FGF-2 and RGP was more obvious (P<0.05). Compared with pretreatment with FGF-2 or RGP alone, pretreatment with LY294002 combined with FGF-2 or RGP significantly down-regulated the expression of cardiac-specific proteins in BMSCs and inhibited the phosphorylation of Akt (P<0.05). Conclusion Both FGF-2 and RGP can induce BMSCs to differentiate into cardiomyocyte-like cells by regulating PI3K/Akt signaling pathway, and the combination of FGF-2 and RGP has a better inductive effect.

Key words

Rehmannia glutinosa polysaccharide

/ Basic fibroblast growth factor / Phosphatiolylinositol 3-kinase/protein kinase B / Bone marrow mesenchymal stem cell / Western blotting / Rat

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LIANG Wei-hong YANG Hui-ying XU Xin-ru SUN Hao-dan LÜ Yang WANG Hai-ping.

Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes induced by rehmannia glutinosa polysaccharide combined with basic fibroblast growth factor

[J]. Acta Anatomica Sinica. 2025, 56(6): 681-687 https://doi.org/10.16098/j.issn.0529-1356.2025.06.007

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