Protective effect of LncRNA SNHG12 on neuronal damage induced by oxygen glucose deprivation/reoxygenation by targeting miR-140-3p

TANG  Chao1  CAO  Yan-qun2*  CHEN  Chao-liang3  ZHOU  Ju-xiang3

doi:10.16098/j.issn.0529-1356.2025.06.004

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Acta Anatomica Sinica ›› 2025, Vol. 56 ›› Issue (6) : 658-663. DOI: 10.16098/j.issn.0529-1356.2025.06.004

Protective effect of LncRNA SNHG12 on neuronal damage induced by oxygen glucose deprivation/reoxygenation by targeting miR-140-3p

TANG Chao¹ CAO Yan-qun^2* CHEN Chao-liang³ ZHOU Ju-xiang³

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Abstract

Objective To investigate the protective effect of long non-coding RNA (lncRNA) small nucleolar RNA host gene 12 (SNHG12) on neuronal damage induced by oxygen glucose deprivation/reoxygenation (OGD/R) by targeting microRNA (miR)-140-3p. Methods Human cortical neurons (HCN) were used to establish an OGD/R model, which was separated into OGD/R group, negative control (NC) group, SNHG12 group, SNHG12+miR-NC group, and SNHG12+miR-140-3p mimics group. Normal cultured HCN were also taken as the control (Ctrl) group. There were 6 repetitions in each group. Cell proliferation, apoptosis, lactate dehydrogenase (LDH) activity, mitochondrial membrane potential, lncRNA SNHG12 and miR-140-3p expression levels, and the interaction of lncRNA SNHG12 and miR-140-3p were detected. Results Compared with the control group, the survival rate of HCN, mitochondrial membrane potential, and lncRNA SNHG12 expression were lower in the OGD/R group, while the LDH activity, apoptosis rate, and miR-140-3p expression were higher (P<0.05). Compared with the OGD/R group and NC group, the survival rate of HCN, mitochondrial membrane potential, and lncRNA SNHG12 expression were higher in the SNHG12 group, while the LDH activity, apoptosis rate, and miR-140-3p expression were lower (P<0.05). Compared with the SNHG12+miR-NC group, the survival rate of HCN, mitochondrial membrane potential, and lncRNA SNHG12 expression were lower in the SNHG12+miR-140-3p mimics group, while the LDH activity, apoptosis rate, and miR-140-3p expression were higher (P<0.05). Dual luciferase activity showed a targeted relationship between lncRNA SNHG12 and miR-140-3p (P<0.05). Conclusion LncRNA SNHG12 may exert a protective effect against OGD/R-induced neuronal damage by inhibiting miR-140-3p.

Key words

Long non-coding RNA

/ Small nucleolar RNA host gene 12 / MicroRNA-140-3p / Oxygen glucose deprivation/reoxygenation / Human cortical neuron

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TANG Chao CAO Yan-qun CHEN Chao-liang ZHOU Ju-xiang. Protective effect of LncRNA SNHG12 on neuronal damage induced by oxygen glucose deprivation/reoxygenation by targeting miR-140-3p[J]. Acta Anatomica Sinica. 2025, 56(6): 658-663 https://doi.org/10.16098/j.issn.0529-1356.2025.06.004

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