Combination of electroencephalogram/electromyography and fiber photometry recording of multiple brain regions in mice and its application in epilepsy models

doi:10.16098/j.issn.0529-1356.2025.05.004

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Acta Anatomica Sinica ›› 2025, Vol. 56 ›› Issue (5) : 533-540. DOI: 10.16098/j.issn.0529-1356.2025.05.004

Combination of electroencephalogram/electromyography and fiber photometry recording of multiple brain regions in mice and its application in epilepsy models

GAO Tong-tong^1,2 CHEN Chen³ YE Wei-yi² DENG Mao² HOU Heng-hao² CHEN Qian^1,2,3*

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Abstract

Objective To develop a method of combining electroencephalogram(EEG) /electromyography (EMG) with multi-regional fiber photometry recording to simultaneously capture the changes of neuronal activity in the whole brain and specific brain regions during epileptic seizures. Methods The mouse head was divided into left and right regions based on the middle suture of the skull. EEG electrodes (EEG/EMG) were implanted in one side, while optical fibers were implanted in the striatum, hippocampus, entorhinal cortex, and thalamus on the contralateral side to simultaneously monitor EEG, EMG, and calcium signal dynamics. Results By combining EEG/EMG with multi-regional fiber photometry recording, differences in neuronal activity across brain regions, alongside EEG and EMG, were observed during different behavioral states. In a kainic acid (KA)-induced epilepsy model, abnormal synchronous neuronal discharges in the mouse brain were accompanied by calcium signal changes in the striatum, hippocampus, entorhinal cortex, and thalamus, with the earliest changes occurring in the hippocampus. Conclusion The combined use of EEG/EMG and multi-brain-region fiber photometry is successfully implemented in mice. This method synchronously recordes abnormal calcium signal changes across multiple brain regions, along with EEG and EMG, in the KA-induced epilepsy model.

Key words

Electrical signal / Calcium signal / Epilepsy / Electroencephalogram / Fiber photometry / Mouse

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GAO Tong-tong CHEN Chen YE Wei-yi DENG Mao HOU Heng-hao CHEN Qian.

Combination of electroencephalogram/electromyography and fiber photometry recording of multiple brain regions in mice and its application in epilepsy models

[J]. Acta Anatomica Sinica. 2025, 56(5): 533-540 https://doi.org/10.16098/j.issn.0529-1356.2025.05.004

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