Construction and evaluation of intestinal mucosal epithelial-myofibroblast co-culture model

doi:10.16098/j.issn.0529-1356.2025.04.008

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Acta Anatomica Sinica ›› 2025, Vol. 56 ›› Issue (4) : 438-443. DOI: 10.16098/j.issn.0529-1356.2025.04.008

Cell and Molecules Biology

Construction and evaluation of intestinal mucosal epithelial-myofibroblast co-culture model

YU Peng-xin WANG Ming-qi GUO Li-na YU Yu WANG Xiu-li^*

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Abstract

Objective To construct a three-dimensional bionic model of intestinal mucosal structure in vitro. Methods Using fibroin protein with villous-crypt structure as scaffold and Caco-2 and HT29-MTX-E12 as seed cells, the intestinal mucosal epithelial-myofibroblast co-culture model was constructed by three-dimensional co-culture with myofibroblasts in vitro. The growth, activity, histological characteristics and expression of functional genes of model cells were investigated through the detection of cell signature proteins and genes. Results 1. An intestinal mucosal epithelial-myofibroblast co-culture model was successfully constructed with good cell activity. 2. Mucous secretion increased and muc2 gene expression was up-regulated in the co-culture model. Conclusion The co-culture of intestinal mucosal epithelial-myofibroblasts with villous-crypt structure can promote the functional differentiation of intestinal epithelial cells and better simulate the structure and function of intestinal mucosa in vivo.

Key words

Intestinal mucosal epithelium model / Fibroin / Villous-crypt / Three-dimensional co-culture

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YU Peng-xin WANG Ming-qi GUO Li-na YU Yu WANG Xiu-li. Construction and evaluation of intestinal mucosal epithelial-myofibroblast co-culture model[J]. Acta Anatomica Sinica. 2025, 56(4): 438-443 https://doi.org/10.16098/j.issn.0529-1356.2025.04.008

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