Objective  To explore an experimental protocol for differentiating humaninduced pluripotent stem cells (iPSCs) into highly pure midbrain dopaminergic (DA) neurons.    Methods  By optimizing a blend of small molecules and recombinant human growth factors, iPSCs were induced to differentiate into ventral midbrain floor plate DA progenitor cells and subsequently into mature substantia nigra pars compacta DA neurons. Throughout the differentiation process, Real-time PCR and immunofluorescent staining were utilized as a method  for quality assessment. Results  iPSCs firstly differentiate into dopaminergic precursor cells, and then gradually differentiate into DA neurons expressing tyrosine hydroxylase (TH).     Conclusion  The protocol successfully yields approximately high purity tyrosine hydroxylase-positive (TH+) DA neurons. This differentiation technique offers an effective cellular model for studying the physiological mechanisms and pathogenesis of Parkinson’s disease, providing valuable insights for future research and potential therapeutic strategies.