Mechanistic of curcumin I in alleviating bleomycin-inducedb pulmonary fibrosis in mice

doi:10.16098/j.issn.0529-1356.2025.02.014

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Acta Anatomica Sinica ›› 2025, Vol. 56 ›› Issue (2) : 232-238. DOI: 10.16098/j.issn.0529-1356.2025.02.014

Histology and Embryology and Development Biology

Mechanistic of curcumin I in alleviating bleomycin-inducedb pulmonary fibrosis in mice

LIN Xin-yi^1,2 TAO Wu-cheng^1*

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Abstract

Objective To explore the effects and mechanisms of curcuminⅠ(CurⅠ)in alleviating bleomycin(BLM)induced pulmonary fibrosis in mice. Methods C57BL/6 mice were randomly divided into 3 groups (n=6 per group), normal group received a single intratracheal instillation of saline; BLM group received a single intratracheal instillation of BLM（3mg/kg）to induce pulmonary fibrosis; BLM+CurⅠgroup received intraperitoneal injections of curcuminⅠ（200μg/kg，Bid×14 day）in addition to BLM treatment. Mice from each group were euthanized on day 14 of the experiment, and lungs, plasma, and bronchoalveolar lavage fluid were collected. Histopathological examination, scoring of pulmonary inflammation and fibrosis were performed to analyze the effects of curcuminⅠon nuclear lung structure in the pulmonary fibrosis mouse model. Immunohistochemistry, Western blotting, and Real-time PCR were conducted to assess the impact of curcuminⅠon nuclear factor(NF)-κB pathway-related protein expression in the pulmonary fibrosis mouse model. ELISA was used to evaluate the effects of curcuminⅠon inflammatory factors in serum and bronchoalveolar lavage fluid of the pulmonary fibrosis mice. Results Compared to the BLM group, intervention with curcuminⅠsignificantly alleviated lung tissue damage in mice. Compared to the normal group, mice in the BLM group showed significantly elevated levels of interleukin(IL)-6 in serum and IL-1β, IL-6 in bronchoalveolar lavage fluid (P<0.001). In contrast, mice in the BLM+CurⅠgroup exhibited significant decreases in serum IL-6 and IL-1β, as well as IL-6 expression in bronchoalveolar lavage fluid (P<0.05). Compared to the normal group, protein expression levels of NF-κB, CD54, growth regulated oncogene (GRO), and CD11b were significantly upregulated in the BLM group (P<0.05). In contrast, protein levels of NF-κB and CD54 were significantly decreased in lung tissues of mice from the BLM+CurⅠgroup (P<0.05), with CD11b and GRO also showing a decreasing trend. Conclusion CurcuminⅠcan suppress inflammation in pulmonary fibrosis mice, improve lung fibrosis, and its mechanism may be associated with modulation of the NF-κB/CD54 signaling pathway.

Key words

CurcuminⅠ

/ Bleomycin / Pulmonary fibrosis / Nuclear factor κB/CD54 signaling pathway / Western blotting / Mouse

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LIN Xin-yi TAO Wu-cheng. Mechanistic of curcumin I in alleviating bleomycin-inducedb pulmonary fibrosis in mice[J]. Acta Anatomica Sinica. 2025, 56(2): 232-238 https://doi.org/10.16098/j.issn.0529-1356.2025.02.014

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