Effect of Draxin on the migration characteristics of trunk neural crest cell in the embryonic mouse spinal cord

doi:10.16098/j.issn.0529-1356.2025.02.004

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Acta Anatomica Sinica ›› 2025, Vol. 56 ›› Issue (2) : 150-157. DOI: 10.16098/j.issn.0529-1356.2025.02.004

Effect of Draxin on the migration characteristics of trunk neural crest cell in the embryonic mouse spinal cord

CUI Zuqi¹ MIAO Xiao-jin² GU Ze-lin² GONG Meng-fei² CHEN Huan² YANG Shu-han² LIU Tong-yu² ZHANG San-bing^3* SU Yu-hong^2*

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Abstract

Objective To investigate the effect of dorsal repulsive axon guidance protein（Draxin）on the migration of trunk neural crest cells during the early development of embryonic mouse spinal cord. Methods Immunohistochemistry and in situ hybridization were used to detect the expression characteristics of Draxin in early embryonic spinal cord (8 mice each group); In situ hybridization was used to detect the change of migration characteristics of trunk neural crest cells in early embryonic spinal cord of different types of mouse (5 mice each group); in vitro culture method was used to check the effect of Draxin on the migration characteristics of embryonic mouse trunk neural crest cells (16 mice each group). Results β-galactosidase gene Z(LacZ) gene was introduced when Draxin gene was knocked out to produce Draxin gene knockout mice. β-galactosidase staining was used to detect LacZ gene expression in Draxin knockout embryonic mice, and the result showed that Draxin expression was observed in the spinal cord of early embryonic mice since 9.5 days(E9.5). Draxin expression was obvious in the embryonic mice spinal cord in E10.5 period. In situ hybridization was used to detect the expression of Draxin gene in the spinal cord of wild type embryonic mice, and the result further verified the obvious expression of Draxin in the early embryonic mice spinal cord in E10.5 period. Sox10 in situ hybridization was used to detect neural crest cell migration in the spinal cord of embryonic mice in E10.5 period. The result showed that segmental migration of neural crest cells in the early embryonic spinal cord of some Draxin knockout mice was delayed compared with the wild type mice. The effect of Draxin on the migration of wild type early embryonic mice trunk neural crest cells in vitro was tested. The result showed that Draxin reduced the migration distance of neural crest cells in vitro. Conclusion In the early developmental stage of embryonic spinal cord (E9.5-E10.5), neural crest cells migrated exuberant. At the same time, Draxin plays an important inhibitory function in the formation of the specific migration pathways of trunk neural crest cells by promoting neural crest cells migrating away from Draxin expressing regions.

Key words

Dorsal repulsive axon guidance protein / Neural crest / Migration / Spinal cord / Immunohistochemistry / Embryonic mice

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CUI Zuqi MIAO Xiao-jin GU Ze-lin GONG Meng-fei CHEN Huan YANG Shu-han LIU Tong-yu ZHANG San-bing SU Yu-hong. Effect of Draxin on the migration characteristics of trunk neural crest cell in the embryonic mouse spinal cord[J]. Acta Anatomica Sinica. 2025, 56(2): 150-157 https://doi.org/10.16098/j.issn.0529-1356.2025.02.004

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