Non-structural maintenance of chromosome condensin Ⅰ complex subunit G promoting the proliferation, migration and invasion of ovarian cancer cells by the Akt signaling pathway

ZHANG Ming-Shu; WANG Yi-Hui; ZHANG Qing; YE Li-Ping

doi:10.16098/j.issn.0529-1356.2024.05.008

PDF(4692 KB)

Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (5) : 573-581. DOI: 10.16098/j.issn.0529-1356.2024.05.008

Non-structural maintenance of chromosome condensin Ⅰ complex subunit G promoting the proliferation, migration and invasion of ovarian cancer cells by the Akt signaling pathway

ZHANG Ming-shu^1,3 WANG Yi-hui¹ ZHANG Qing¹ YE Li-ping^1,2*

Author information +

History +

Abstract

Objective To explore the regulatory mechanism of non-structural maintenance of chromosome condensin I complex submit G (NCAPG) on proliferation, migration, and invasion of ovarian cancer cells. Methods The bioinformatics database was used to analyze the differential expression of NCAPG in ovarian cancer tissues. Western blotting was used to detect the protein expression of NCAPG in normal ovarian epithelial cells IOSE80, ovarian cancer A2780 and SKOV3 cells. The silencing experiments of NCAPG siRNA were divided into blank, control, siNCAPG-1 and siNCAPG-2 groups. The overexpression experiments of NCAPG plasmid were divided into blank, control, NCAPG, NCAPG+MK2206 and MK2206 groups. MTT assay was used to detect cell proliferation activity. Cell scoring assay and transwell assay were used to analyze cell migration and invasion. The protein expressions of p-Akt, total(t)-Akt, proliferative cellular nucleus antigen (PCNA), matrix metallopeptidase 9 (MMP-9), vimentin, N-cadherin, and E-cadherin were detected by Western blotting. Results NCAPG was highly expressed in ovarian cancer tissues and cells. Knockdown of NCAPG significantly inhibited the proliferation, invasion and migration of ovarian cancer SKOV3 cells. The protein expressions of p-Akt, PCNA, MMP-9, vimentin and N-cadherin decreased while E-cadherin expression increased. Overexpression of NCAPG significantly promoted the proliferation, invasion and migration of ovarian cancer A2780 cells. The protein expressions of pAkt, PCNA, MMP-9, vimentin, and N-cadherin increased while E-cadherin expression decreased. Akt inhibitor MK2206 significantly attenuated the above effects of NCAPG. Conclusion NCAPG promotes the proliferation, invasion, and migration of ovarian cancer cells by activating the Akt signaling pathway and regulating the expression of PCNA, MMP-9, and epithelial-mesenchymal transition (EMT)-related proteins.

Key words

/ "> Ovarian cancer｜Non-structural maintenonce of chromosome condensin Ⅰ complex subunit G｜Protein kinase B signaling pathway｜Proliferatiing cell nuclear antigen｜Matrix metallopeptidase 9｜Cadherin｜Western blotting

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHANG Ming-shu WANG Yi-hui ZHANG Qing YE Li-ping. Non-structural maintenance of chromosome condensin Ⅰ complex subunit G promoting the proliferation, migration and invasion of ovarian cancer cells by the Akt signaling pathway[J]. Acta Anatomica Sinica. 2024, 55(5): 573-581 https://doi.org/10.16098/j.issn.0529-1356.2024.05.008

References

［1］ Moufarrij S, Dandapani M, Arthofer E, et al.Epigenetic therapy for ovarian cancer: promise and progress［J］.Clin Epigenetics,2019, 11 (1): 7.

［2］ Sutani T, Sakata T, Nakato R, et al.Condensin targets and reduces unwound DNA structures associated with transcription in mitotic chromosome condensation［J］.Nat Commun,2015, 6: 7815.

［3］ Jiang L, Ren L, Chen H, et al.NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer［J］.Cell Death Dis,2020, 11 (7): 547.

［4］ Wu Y, Lin Y, Pan J, et al.NCAPG promotes the progression of lung adenocarcinoma via the TGF-beta signaling pathway［J］.Cancer Cell Int,2021, 21 (1): 443.

［5］ Sun DP, Lin CC, Hung ST, et al.Aberrant Expression of NCAPG is associated with prognosis and progression of gastric cancer［J］.Cancer Manag Res,2020, 12 (6): 7837-7846.

［6］ Gong C, Ai J, Fan Y, et al.NCAPG promotes the proliferation of hepatocellular carcinoma through PI3K/AKT signaling［J］.Onco Targets Ther,2019, 12 (1): 8537-8552.

［7］ Zhang X, Zhu M, Wang H, et al.Overexpression of NCAPG inhibits cardia adenocarcinoma apoptosis and promotes epithelial-mesenchymal transition through the Wnt/beta-catenin signaling pathway［J］.Gene,2021, 766 (4): 145-163.

［8］ Palou R, Dhanaraman T, Marrakchi R, et al.Condensin ATPase motifs contribute differentially to the maintenance of chromosome morphology and genome stability［J］.PLoS Biol,2018, 16 (6): e2003980.

［9］ Liu X, Chen Y, Li Y, et al.Targeting mitosis exit: A brake for cancer cell proliferation［J］.Biochim Biophys Acta Rev Cancer,2019, 1871 (1): 179-191.

［10］ Zhang X, Wang H, Han Y, et al.NCAPG induces cell proliferation in cardia adenocarcinoma via PI3K/AKT signaling pathway［J］.Onco Targets Ther,2020, 13 (2): 11315-11326.

［11］ Guo ZY, Zhu ZT. NCAPG is a prognostic biomarker associated with vascular invasion in hepatocellular carcinoma［J］.Eur Rev Med Pharmacol Sci,2021, 25 (23): 7238-7251.

［12］ Yu H, Zou D, Ni N, et al.Overexpression of NCAPG in ovarian cancer is associated with ovarian cancer proliferation and apoptosis via p38 MAPK signaling pathway［J］.J Ovarian Res,2022, 15 (1): 98.

［13］ Ediriweera MK, Tennekoon KH, Samarakoon SR.Role of the PI3K/AKT/mTOR signaling pathway in ovarian cancer: Biological and therapeutic significance［J］.Semin Cancer Biol,2019, 59 (10): 147-160.

［14］ Li P, Wen J, Ren X, et al.MicroRNA23b3p targets nonSMC condensing I complex subunit G to promote proliferation and inhibit apoptosis of colorectal cancer cells via regulation of the PI3K/AKT signaling pathway［J］.Oncol Lett,2021, 22 (6): 812.

［15］ Nowak E, Bednarek I.Aspects of the epigenetic regulation of EMT related to cancer metastasis［J］.Cells,2021, 10 (12): 34-35.

［16］ Yeung KT, Yang J.Epithelial-mesenchymal transition in tumor metastasis［J］.Mol Oncol,2017, 11 (1): 28-39.

［17］ Shi Y, Ge C, Fang D, et al.NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition by activating the Wnt/β-catenin signaling pathway［J］.Cancer Cell Int,2022, 22 (1): 119.

［18］ Huang H.Matrix metalloproteinase-9 (MMP-9) as a cancer biomarker and MMP-9 biosensors: recent advances［J］.Sensors (Basel),2018, 18 (10): 32-49.

［19］ Hu X, Li D, Zhang W, et al.Matrix metalloproteinase-9 expression correlates with prognosis and involved in ovarian cancer cell invasion［J］.Arch Gynecol Obstet,2012, 286 (6): 1537-1543.

［20］ Scheau C, Badarau IA, Costache R, et al.The Role of Matrix metalloproteinases in the epithelial-mesenchymal transition of hepatocellular carcinoma［J］.Anal Cell Pathol (Amst),2019, 2019: 9423907.

［21］ Stoimenov I, Helleday T.PCNA on the crossroad of cancer［J］.Biochem Soc Trans,2009, 37 (Pt 3): 605-613.

［22］ Liu C, Yan Y, Di F, et al.Inhibition of NCAPG expression inactivates the Wnt/beta-catenin signal to suppresses endometrial cancer cell growth in vitro［J］.Environ Toxicol,2021, 36 (12): 2512-2520.

［23］ Peng B, Ortega J, Gu L, et al.Phosphorylation of proliferating cell nuclear antigen promotes cancer progression by activating the ATM/Akt/GSK3beta/Snail signaling pathway［J］.J Biol Chem,2019, 294 (17): 7037-7045.

［24］ Zhao FJ, Kang CS, Cui XW, et al.［The relationship of MMP-9, VEGF and PCNA expressions and their clinical significance in gastric adenocarcinoma［J］.Zhonghua Nei Ke Za Zhi,2009, 48 (2): 114-117.

［25］ Suman S, Kurisetty V, Das TP, et al.Activation of AKT signaling promotes epithelial-mesenchymal transition and tumor growth in colorectal cancer cells［J］.Mol Carcinog,2014, Suppl 1: E151-160.