Effect of sperm DNA fragmentation on in vitro fertilization-embryo&nbsp;transfer outcome among middle-aged men

WANG Ping; WANG Jing; CHEN Xian-Bing; CHEN Xi

doi:10.16098/j.issn.0529-1356.2024.03.013

PDF(2206 KB)

Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (3) : 345-348. DOI: 10.16098/j.issn.0529-1356.2024.03.013

Histology,Embryology and Developmental Biology

Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men

WANG Ping WANG Jing CHEN Xian-bing CHEN Xi*

Author information +

History +

Abstract

Objective To explore the relationship between sperm DNA fragmentation index(DFI) and sperm quality, as well as in vitro fertilization-embryo transfer (IVF-ET) preganancy outcomes in middle-aged men. Methods A total of 180 semen specimens from males aged over 38 years who received comventional IVF-ET treatment were collected and divided into two groups(<30% and ≥30%) based on the DIF threshold. Main measures result including: normal semen parameters, hormone levels, DFI, fertilization rate, good quality embryo rate and pregnancy rate. Results DFI was closely related to male follicle-stimulating hormone（FSH）, sperm motility，morphology, and the sperm vitality decreased with increasing DFI level (P<0.05). When DFI≥30%, the rate of high-quality embryos decreased (P<0.05), but there was no significant difference in the pregnancy rate (P>0.05). Conclusion DFI can serve as an important indicator for routine analysis of semen in middle-aged men. DFI affects the rate of high-quality embryos, but it is not related to the clinical pregnancy outcome of assistant reproduction technology (ART).

Key words

DNA fragmentation index / Sperm motility / Sperm chromatin dispersion / Morphological observation / Human

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WANG Ping WANG Jing CHEN Xian-bing CHEN Xi. Effect of sperm DNA fragmentation on in vitro fertilization-embryo transfer outcome among middle-aged men[J]. Acta Anatomica Sinica. 2024, 55(3): 345-348 https://doi.org/10.16098/j.issn.0529-1356.2024.03.013

References

［1］ Agarwal A, Parekh N, Selvam MK, et al. Male oxidative stress infertility (MOSI): proposed terminology and clinical practice guidelines for management of idiopathic male infertility［J］.World J Mens Health, 2019, 37(3):296-312.

［2］ Hozyen MM, Hassanen EM, Sayed Azzouz Y, et al. Do different sperm selection techniques have an impact on embryological findings and clinical outcomes of abnormal sperm DNA fragmentation patients compared to normal ones, a retrospective cohort study［J］. Fertil Steril, 2019,112(3):e279.

［3］ Jerre E, Bungum M, Evenson D, et al. Sperm chromatin structure assay high DNA stainability sperm as a marker of early miscarriage after intracytoplasmic sperm injection［J］. Fertil Steril, 2019, 112(1):46-53.e2.

［4］ Tello-Mora P, Hernndez-Cadena L, Pedraza J, et al. Acrosome reaction and chromatin integrity as additional parameters of semen analysis to predict fertilization and blastocyst rates［J］. Reprod Biol Endocrinol, 2018, 16(1):102.

［5］ Ni WH, Agarwal A, Sun YP, et al. Clinical expert consensus on sperm DNA fragment detection［J］. Journal of Reproductive Medicine, 2023, 32(2):170-180.(in Chinese)倪吴花, Agarwal A, 孙莹璞, 等. 精子DNA碎片检测的临床专家共识［J］. 生殖医学杂志, 2023, 32(2):170-180.

［6］ Fernández JL, Muriel L, Goyanes V, et al., Halosperm is an easy, available, and cost-effective alternative for determining sperm DNA fragmentation［J］. Fertil Steril, 2005, 84(4):860.

［7］ Li X, Zhu Y, Zhang C, et al. BDE-209 induces male reproductive toxicity via cell cycle arrest and apoptosis mediated by DNA damage response signaling pathways［J］. Environ Pollut, 2019, 255(Pt 1):113097.

［8］ Nicopoullos J, Vicens-Morton A, Lewis SEM, et al. Novel use of COMET parameters of sperm DNA damage may increase its utility to diagnose male infertility and predict live births following both IVF and ICSI［J］. Hum Reprod, 2019, 34(10):1915-1923.

［9］ Evenson, DP. The Sperm Chromatin Structure Assay (SCSA((R))) and other sperm DNA fragmentation tests for evaluation of sperm nuclear DNA integrity as related to fertility［J］. Anim Reprod Sci, 2016, 169:56-75.

［10］ Simon L, Emery B, Carrell DT. Sperm DNA fragmentation: consequences for reproduction［J］. Adv Exp Med Biol,2019,1166:87-105.

［11］ Petersen CG, Mauri AL, Vagnini LD, et al. The effects of male age on sperm DNA damage: an evaluation of 2,178 semen samples［J］. JBRA Assisted Reproduction, 2018,22 (4):323-330.

［12］ Simon L, Aston KI, Emery BR, et al. Sperm DNA damage output parameters measured by the alkaline Comet assay and their importance［J］. Andrologia,2017,49(2):e12608.

［13］ Minhas S, Bettocchi C, Boeri L, et al. European association of urology guidelines on male sexual and reproductive health:2021 update on male infertility［J］. Eur Urol, 2021, 80(5):603-620.

［14］ Simon L, Zini A, Dyachenko A, et al. A systematic review and metaanalysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome［J］. Asian J Androl, 2017,19(1):80-90.

［15］ Carlini T, Paoli D, Pelloni M, et al. Sperm DNA fragmentation in Italian couples with recurrent pregnancy loss［J］. Reprod Biomed Online, 2017,34(1):58-65.

［16］ Uppangala S, Pudakalakatti F, D’Souza F, et al. Influence of sperm DNA damage on human preimplantation embryo metabolism［J］. Reprod Biol, 2016, 16(3): 234-241.

［17］ Fernandez-Diez C, Gonzalez-Rojo S, Lombo M, et al. Impact of sperm DNA damage and oocyte-repairing capacity on trout development［J］. Reproduction, 2016, 152(1):57-67.

［18］ Sastre D, da Costa NN, de Sa AL, et al. Expression of PLIN2 and PLIN3 during oocyte maturation and early embryo development in cattle［J］. Theriogenology, 2014, 81(2):326-331.

［19］ Nasr-Esfahani MH, Salehi M, Razavi S, et al. Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI［J］. Reprod Biomed Online, 2005,11(2):198-205.

［20］ Tesarik J, Greco E, Mendoza C. Late, but not early, paternal effect on human embryo development is related to sperm DNA fragmentation［J］. Hum Reprod, 2004, 19(3):611-615.