Association between index-ring finger length ratio and polymorphisms of 6 phalange-bone development related genes

YANG Meng-Yi; NIU Shi-Bo; ZHANG Jing; PENG Liang; DANG Jie; MA Zhan-Bing; LU Hong; HUO Zheng-Hao

doi:10.16098/j.issn.0529-1356.2024.02.008

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Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (2) : 181-187. DOI: 10.16098/j.issn.0529-1356.2024.02.008

Cell and Molecules Biology

Association between index-ring finger length ratio and polymorphisms of 6 phalange-bone development related genes

YANG Meng-yi NIU Shi-bo ZHANG Jing PENG Liang DANG Jie MA Zhan-bing LU Hong* HUO Zheng-hao*

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Abstract

Objective To investigate the association of 13 single nucleotide polymorphism (SNP) sites in 6 phalange-bone development related genes ［fibroblast growth factor receptor 2 (FGFR2), indian hedgehog signaling molecule (IHH), Msh homeobox 1(MSX1), Runx family transcription factor 2(RUNX2), SRY-box transcription factor 9(SOX9), Wnt family member 5A (WNT5A)］ with human index-ring finger length ratio (2D∶4D). Methods Digital cameras were used to take frontal photographs of the hands of 731 college students (358 males and 373 females) in Ningxia, and image analysis software was used to mark anatomical points and measure finger lengths of index (2th) and ring (4th); genotyping of 13 SNP sites (rs1047057, rs755793, rs41258305, rs3731881, rs3100776, rs12532, rs3821949, rs45585135, rs3749863, rs1042667, rs12601701, rs1829556, rs3732750) for 6 genes by multiplex PCR; One-Way ANOVA or independent sample t-test indirectly assessed the association between 2D∶4D and 13 SNP sites. Results Both left and right hand 2D∶4D were significantly higher in females than males in Ningxia college students (all P<0.01); no statistically significant differences in genotype and allele frequencies of the 13 SNP sites among different sexes (all P>0.05); among different sexes, male left hand 2D∶4D was significantly associated with the genotype of SOX9 gene rs12601701 site (P<0.05) and right hand 2D∶4D was significantly associated with the genotype of WNT5A gene rs1829556 site (P<0.05); the female right hand 2D∶4D was significantly associated with the MSX1 gene rs12532 (P<0.01) and rs3821949 (P<0.05) sites genotypes. Conclusion SOX9 (rs12601701), WNT5A (rs1829556) and MSX1 (rs12532 and rs3821949) gene polymorphisms may be associated with the formation of 2D∶4D in Ningxia population.

Key words

Phalange-bone development gene / Msh homeobox 1 gene / SRY-box transcription factor 9 gene / Wnt family member 5A gene / Finger length ratio / Multiplex PCR / Ningxia / College student

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YANG Meng-yi NIU Shi-bo ZHANG Jing PENG Liang DANG Jie MA Zhan-bing LU Hong HUO Zheng-hao. Association between index-ring finger length ratio and polymorphisms of 6 phalange-bone development related genes[J]. Acta Anatomica Sinica. 2024, 55(2): 181-187 https://doi.org/10.16098/j.issn.0529-1356.2024.02.008

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