Scutellarin inhibitting BV-2 microglia-mediated neuroinflammation via the cyclic GMP-AMP synthase - stimulator of interferon gene  pathway

DUAN Zhao-Da; YANG Li; CHEN Hao-Lun; LIU Teng-Teng; ZHENG Li-Yang; XU Dong-Yao; WU Chun-Yun

doi:10.16098/j.issn.0529-1356.2024.02.002

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Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (2) : 133-142. DOI: 10.16098/j.issn.0529-1356.2024.02.002

Scutellarin inhibitting BV-2 microglia-mediated neuroinflammation via the cyclic GMP-AMP synthase - stimulator of interferon gene pathway

DUAN Zhao-da YANG Li CHEN Hao-lun LIU Teng-teng ZHENG Li-yang XU Dong-yao WU Chun-yun*

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Abstract

Objective To explore the effect of scutellarin on lipopolysaccharide (LPS) induced neuroinflammation in BV-2 microglia cells. Methods BV-2 microglia were cultured and randomly divided into 6 groups: control group (Ctrl), cyclic GMP-AMP synthetase（cGAS）inhibitor RU320521 group (RU.521 group), LPS group, LPS+RU.521 group, LPS+ scutellarin pretreatment group (LPS+S) and LPS+S+RU.521 group. The expressions of cGAS, stimulator of interferon gene (STING), nuclear factor kappa B (NF-κB), phosphorylated NF-κB (p-NF-κB), neuroinflammatory factors PYD domainscontaining protein 3（NLRP3) and tumor necrosis factor α（TNF-α）in BV-2 microglia were detected by Western blotting and immunofluorescent double staining (n=3). Results Western blotting and immunofluorescent double staining showed that compared with the control group, the expression of cGAS, STING, p-NF-κB, NLRP3 and TNF-α in BV-2 microglia increased significantly after LPS induction(P＜0.05), while the expression of cGAS, STING, p-NF-κB, NLRP3 and TNF-α in LPS+S group were significantly lower than those in LPS group (P＜0.05). Treatment with cGAS pathway inhibitor RU.521 showed similar effects as the pre-treatment group with scutellarin. In addition, the change of NF-κB in each group was not statistically significant（P＞0.05）. Conclusion Scutellarin inhibits the neuroinflammation mediated by BV-2 microglia cells, which may be related to cGAS-STING signaling pathway.

Key words

Scutellarin / BV-2 microglia / Cyclic GMP-AMP synthetase-stimulator of interferon gene pathway / PYD domains-containing protein3 / Neuroinflammation / Immunofluorescence / Western blotting

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DUAN Zhao-da YANG Li CHEN Hao-lun LIU Teng-teng ZHENG Li-yang XU Dong-yao WU Chun-yun. Scutellarin inhibitting BV-2 microglia-mediated neuroinflammation via the cyclic GMP-AMP synthase - stimulator of interferon gene pathway[J]. Acta Anatomica Sinica. 2024, 55(2): 133-142 https://doi.org/10.16098/j.issn.0529-1356.2024.02.002

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