Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method

HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe

Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (1) : 120-124.

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Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (1) : 120-124. DOI: 10.16098/j.issn.0529-1356.2024.01.017
Technology and Methodology

 Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method

  •  HAO  Yong-ming1  GUO  Lei2  ZHOU  Cheng-hui PEI  Han-jun1*  LI  Li1  ZHAO  Zhe1
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Abstract

Objective  To compare effectiveness between the modified and traditional pressure-overload myocardial hypertrophy(POMH) model by abdominal aorta coarctation(AAC) method.    Methods  Totally 45 rats were divided into three groups(n=15 per group), sham group, traditional group, and modified group. In the traditional group, the diameter of the abdominal aorta was narrowed to 0.70 mm through a midline incision for 4 weeks; in the modified group, the diameter of the abdominal aorta was narrowed above the left kidney to 0.45 mm for 1 week, and then the narrowing was lifted postoperatively. The cardiac index, heart weight(HW)/body weight (BW) and left ventricular index, left ventricular weight(LVW)/BW were measured from the heart specimens, and the cross-sectional area of cardiac myocytes, myocardial collagen area, and myocardial collagen area fraction were measured in the pathological sections by HE staining and Masson staining.    Results  Compared with the sham group, the differences in end-systolic interventricular septum thickness(IVSs), left ventricular end-systolic posterior wall thickness (LVPWs), HW/BW, LVW/BW, cardiomyocyte cross-sectional area, myocardial collagen area, myocardial collagen area fraction, and brain natriuretic peptide (BNP) expression levels were statistically significant (P<0.05) in the modified and traditional groups of rats. The differences in these indices were not statistically significant between the modified and traditional groups (P>0.05).    Conclusion  The modified abdominal aortic constriction method  used in this experiment is time-saving, stable, homogeneous and easy to replicate, and is a more ideal approach to establish a rat model of POMH.

Key words

 Myocardial hypertrophy / Modified abdominal aortic coarctation method /   / Echocardiography / Western blotting / Rat
 

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HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe.  Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method[J]. Acta Anatomica Sinica. 2024, 55(1): 120-124 https://doi.org/10.16098/j.issn.0529-1356.2024.01.017

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