PDF(12732 KB)
Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia
WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen
Acta Anatomica Sinica ›› 2024, Vol. 55 ›› Issue (1) : 3-9.
PDF(12732 KB)
PDF(12732 KB)
Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia
Objective To observe the expression and localization of group Ⅰ metabotropic glutamate receptors (mGluR1/5) in rat superior cervical ganglion (SCG) and the effect of chronic intermittent hypoxia (CIH) on mGluR1/5 protein level. Methods Twelve male SD rats were randomly divided into control group（Ctrl）and CIH group（CIH）, 6 rats in each group. After 6 weeks of modeling, the effect of CIH on mGluR1/5 protein level was detected by Western blotting, the expression and distribution of mGluR1/5 in SCG were detected by immunohistochemistry and double-immunofluorescent staining. Results mGluR1/5 was expressed in rat SCG. mGluR1 was distributed in neurons and small intensely fluorescent (SIF) cells, but not in satellite glial cells (SGCs), nerve fibers and blood vessels, whereas mGluR5 was mainly distributed in nerve fibers and a little in neurons, but not in SGCs, SIF cells and blood vessels. CIH increased the protein levels of mGluR1/5 (P<0.01) in rat SCG. Conclusion Both mGluR1 and mGluR5 are expressed in the rat SCG, but their distribution are different, and the increased protein levels of both may be involved in CIH-induced hypertension.
Group Ⅰ metabotropic glutamate receptor
［1］Niswender CM, Conn PJ. Metabotropic glutamate receptors: physiology, pharmacology, and disease ［J］. Annu Rev Pharmacol Toxicol, 2010, 50:295-322.
［2］Reiner A, Levitz J. Glutamatergic signaling in the central nervous system: ionotropic and metabotropic receptors in concert ［J］. Neuron, 2018, 98(6):1080-1098.
［3］Sladeczek F, Momiyama A, Takahashi T. Presynaptic inhibitory action of a metabotropic glutamate receptor agonist on excitatory transmission in visual cortical neurons ［J］. Proc Biol Sci, 1993, 253(1338):297-303.
［4］Gereau RW 4th, Conn PJ. Multiple presynaptic metabotropic glutamate receptors modulate excitatory and inhibitory synaptic transmission in hippocampal area CA1 ［J］. J Neurosci, 1995, 15(10):6879-6889.
［5］Sheng N, Yang J, Silm K, et al. A slow excitatory postsynaptic current mediated by a novel metabotropic glutamate receptor in CA1 pyramidal neurons ［J］. Neuropharmacology, 2017, 115:4-9.
［6］Jin LE, Wang M, Galvin VC, et al. mGluR2 versus mGluR3 metabotropic glutamate receptors in primate dorsolateral prefrontal cortex: postsynaptic mGluR3 strengthen working memory networks ［J］. Cereb Cortex, 2018, 28(3):974-987.
［7］Salman LA, Shulman R, Cohen JB. Obstructive sleep apnea, hypertension, and cardiovascular risk: epidemiology, pathophysiology, and management ［J］. Curr Cardiol Rep, 2020, 22(2):6-15.
［8］Wszedybyl-Winklewska M, Wolf J, Szarmach A, et al. Central sympathetic nervous system reinforcement in obstructive sleep apnoea ［J］. Sleep Med Rev, 2018, 39:143-154.
［9］Li C, Zhao B, Fan YN, et al. Expression of BACE1 in the Rat Carotid Body ［J］. Front Physiol, 2020, 11:505.
［10］García-Bea A, Walker MA, Hyde TM, et al. Metabotropic glutamate receptor 3 (mGlu3; mGluR3; GRM3) in schizophrenia: antibody characterisation and a semi-quantitative western blot study ［J］. Schizophr Res, 2016, 177(1-3):18-27.
［11］Traynelis SF, Wollmuth LP, McBain CJ, et al. Glutamate receptor ion channels: structure, regulation, and function ［J］. Pharmacol Rev, 2010, 62(3):405-496.
［12］Tremolizzo L, Sala G, Zoia CP, et al. Assessing glutamatergic function and dysfunction in peripheral tissues ［J］. Curr Med Chem, 2012, 19(9):1310-1315.
［13］Chen TJ, Kukley M. Glutamate receptors and glutamatergic signalling in the peripheral nerves ［J］. Neural Regen Res, 2020, 15(3):438-447.
［14］Kiyama H, Sato K, Kuba T, et al. Sympathetic and parasympathetic ganglia express non-NMDA type glutamate receptors: distinct receptor subunit composition in the principle and SIF cells ［J］. Brain Res Mol Brain Res, 1993, 19(4):345-348.
［15］Ito T, Iino S, Nojyo Y. A part of cholinergic fibers in mouse superior cervical ganglia contain GABA or glutamate ［J］. Brain Res, 2005, 1046(1-2):234-238.
［16］Ikeda SR, Lovinger DM, McCool BA, et al. Heterologous expression of metabotropic glutamate receptors in adult rat sympathetic neurons: subtype-specific coupling to ion channels ［J］. Neuron, 1995, 14(5):1029-1038.
［17］Kammermeier PJ, Ikeda SR. Metabotropic glutamate receptor expression in the rat superior cervical ganglion ［J］. Neurosci Lett, 2002, 330(3):260-264.
［18］Senba E, Kaneko T, Mizuno N, et al. Somato-, branchio-and viscero-motor neurons contain glutaminase-like immunoreactivity ［J］. Brain Res Bull, 1991, 26(1):85-97.
［19］Pan ZQ, Pan XD. Forty years of research on cervicogenic hypertension［C］. Proceedings of the Eighth National Conferencen on Cervial Spandylosis of Chinese Association of Rehabilitation Medicine, 2004, 4-7. (in Chinese)
潘之清，潘旭东．颈源性高血压四十年研究 ［C］. 中国康复医学会第8次全国颈椎学术会议论文集，2004，4-7.
［20］Liu HT. Research progress on the relationship between cervical spine and hypertension ［J］. Journal of Practical Cardiovascular and Cerebrovascular Diseases. 2012, 20(9):1214-1244. (in Chinese)
刘洪涛．颈椎部位与高血压相关性的研究进展 ［J］. 实用心脑肺血管病杂志, 2012, 20(9):1214-1244.
［21］He ZB, Lu YK, Chen DCh. Overview of domestic research on abnormal cervical blood pressure ［J］. Chinese Journal of Physical Medicine and Rehabilitation, 2006, 9(28):628-637. (in Chinese)
何宗宝，吕有魁，陈东昌．颈椎性血压异常国内研究概况 ［J］. 中华物理医学与康复杂志, 2006, 9(28):628-637.
［22］Miniaci MC, Bonsi P, Tempia F, et al. Presynaptic modulation by group Ⅲ metabotropic glutamate receptors (mGluRs) of the excitatory postsynaptic potential mediated by mGluR1 in rat cerebellar Purkinje cells ［J］. Neurosci Lett, 2001, 310(1):61-65.
［23］Battaglia G, Bruno V, Pisani A, et al. Selective blockade of type-1 metabotropic glutamate receptors induces neuroprotection by enhancing gabaergic transmission ［J］. Mol Cell Neurosci, 2001, 17(6):1071-1083.
［24］Dobó E, Kása P, Joó F, et al. Structures with GABA-like and GAD-like immunoreactivity in the cervical sympathetic ganglion complex of adult rats ［J］. Cell Tissue Res, 1990, 262(2):351-361.
［25］Dobó E, Joó F, Wolff JR. Distinct subsets of neuropeptide Y-negative principal neurons receive basket-like innervation from enkephalinergic and gabaergic axons in the superior cervical ganglion of adult rats ［J］. Neuroscience, 1993, 57(3):833-844.
［26］We XX, Li ChH, Zhao ChL, et al. Characterization of metabotropic glutamate receptor 7 and 8 in rat superio cervicalganglion and their changes following chrnic intermittent hypoxia［J］. Journal of Southern Medical University, 2023, 43(7): 172-1178. (in Chinese)
魏茜茜，李超红，赵晨露，等. 大鼠颈上神经节代谢型谷氨酸受体7、8的表达及慢性间歇性低氧对其的影响［J］. 南方医科大学学报, 2023, 43(7): 172-1178.
PDF(12732 KB)
/
| 〈 |
|
〉 |
