Structural distribution and
mechanism of elastic fibers and collagen fibers in ventricular interstitium of
aged rats

XU Ya-Ping; YU Yue-Xin; CHEN Jin-Fu; WANG Ke-Ke; GUO Zhi-Kun

doi:10.16098/j.issn.0529-1356.2023.06.014

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (6) : 716-721. DOI: 10.16098/j.issn.0529-1356.2023.06.014

Histology,Embryology and Developmental Biology

Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats

XU Ya-ping¹ YU Yue-xin¹ CHEN Jin-fu² WANG Ke-ke¹ GUO Zhikun^1,2*

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Abstract

Objective To investigate the structural distribution features and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats. Methods Five young SD rats (24 weeks) and five old SD rats (104 weeks) were used，and their cardiac function was examined by echocardiography. Modified Weigert elastic fiber staining, immunohistochemistry, immunofluorescence and Western blotting techniques were used to detect the expression changes of type I and Ⅲ collagen fibers and their proteins, elastic fibers and their proteins, matrix metalloproteinase 2 (MMP-2), matrix metalloproteinase 9 (MMP-9) and tissue inhibitor of metalloproteinase 2 (TIMP-2), respectively. Results The type I and type Ⅲ collagen in the ventricular interstitium of aged rats was very sufficient and wrapped around the cardiomyocytes. Compared with the young rats, the content of collagen protein in the ventricular interstitium of the aged rats significantly increased (P<0.05). Elastic fibers in the ventricular interstitium of the aged rats were and widely distributed. Compared with the young rats, the number of elastic fibers and the level of elastin in the ventricular interstitium of the aged rats significantly decreased (P<0.05), and the expression levels of MMP-2 and MMP-9 in ventricular muscle of aged rats increased, and they were correlated with the level of elastin. The level of TIMP-2 in ventricular muscle of aged rats decreased with age. Conclusion The number of collagen fibers and elastic fibers in ventricular interstitium of aged rats is fluctuated with each other. With the increase of age, the contents of TIMP-2 and elastic fibers in the ventricular interstitium gradually decreased, and the ratio of collagen fibers to elastic fibers is out of balance.

Key words

Matrix metalloproteinase 2 / Matrix metalloproteinase 9 / Tissue inhibitor of metalloproteinase 2 / Collagen fiber / Elastic fiber / Ventricular muscle / Immunofluorescence / Western blotting / Rat

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XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun. Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats[J]. Acta Anatomica Sinica. 2023, 54(6): 716-721 https://doi.org/10.16098/j.issn.0529-1356.2023.06.014

References

［1］Li L, Zhao Q, Kong W. Extracellular matrix remodeling and cardiac fibrosis［J］. Matrix Biol, 2018, 68-69:490-506.

［2］Meschiari CA, Ero OK, Pan HH, et al. The impact of aging on cardiac extracellular matrix［J］. Geroscience, 2017,39(1):7-18.

［3］Miekus N, Luise C, Sippl W, et al. MMP-14 degrades tropoelastin and elastin ［J］. Biochimie, 2019, 165:32-39.

［4］El-Busaid H, Hassan S, Odula P, et al. Sex variations in the structure of human atrioventricular annuli［J］. Folia Morphol (Warsz), 2012, 71(1):23-27.

［5］Zhai XB, Wu JF, Guo ZhK, et al. Aging changes of elastic fibers and elastin in normal rat ventricular muscle［J］. Acta Anatomica Sinica, 2013，44(1): 106-110．(in Chinese)

翟新博，武俊芳，郭志坤，等．正常大鼠心室肌弹性纤维和弹性蛋白的增龄变化［J］. 解剖学报，2013，44(1): 106-110.

［6］Shi XY. Medical Experimental Zoology［M］. Beijing: People’s Military Medical Publishing House, 1999: 388. (in Chinese)

施新猷. 医用实验动物学［M］.北京:人民军医出版社，1999: 388.

［7］Biernacka A，Frangogiannis NG．Aging and cardiac fibrosis［J］. Aging Dis, 2011，2(2): 158-173.

［8］Yu Y，Yin G，Bao S，et al．Kinetic alterations of collagen and elastic fibers and their association with cardiac function in acute myocardial infarction［J］. Mol Med Rep，2018，17 (3):3519-3526.

［9］Mizuno T，Mickle DA，Kiani CG，et al．Overexpression of elastin fragments in infarcted myocardium attenuates scar expansion and heart dysfunction［J］. Am J Physiol Heart Circ Physiol，2005，288(6): H2819-H2827.

［10］Bertram C, Hass R. Cellular senescence of human mammary epithelial cells (HMEC) is associated with an altered MMP-7/HB-EGF signaling and increased formation of elastin-like structures ［J］. Mech Ageing Dev, 2009,130(10):657-669.

［11］Mizuno T, Mi ckle DA, Kiani CG, et al. Overexpression of elastin fragments in infarcted myocardium attenuates scar expansion and heart dysfunction ［J］. Am J Physiol Heart Circ Physiol, 2005, 288 (6): 2819-2827.

［12］Wang KK, Meng XG, Guo ZhK.Elastin structure, synthesis, regulatory mechanism and relationship with cardiovascular diseases ［J］. Front Cell Dev Biol, 2021,9:596702.

［13］Zhong ChH, Cao MJ, Shu M, et al. Tissue inhibitor of metalloproteinase-2 (TIMP-2) from red sea bream (Pagrus major): Molecular cloning and biochemical characterization of highly expressed recombinant protein［J］. Fish Shellfish Immunol, 2019,95:556-563.

［14］Kisling A, Lust RM, Katwa LC. What is the role of peptide fragments of collagen I and IV in health and disease［J ? Life Sci, 2019,228:30-34.

［15］Ninh VK, Hajj EC, Ronis MJ, et al. N-Acetylcysteine prevents the decreases in cardiac collagen I/Ⅲ ratio and systolic function in neonatal mice with prenatal alcohol exposure［J］. Toxicol Lett, 2019,315:87-95.

［16］Gazoti Debessa CR, Mesiano Maifrino LB, Rodrigues de Souza R. Age related changes of the collagen network of the human heart ［J］.Mech Ageing Dev, 2001, 122(10):1049-1058.