Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition

WANG Wen-Juan; DING Yu-Tong; SU Hao; REN Jie; SUN Yan-Rong; LU Jia-Li; ZHANG Lin-Qian; BAI Yu; QIN Li-Hua

doi:10.16098/j.issn.0529-1356.2023.06.001

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (6) : 620-627. DOI: 10.16098/j.issn.0529-1356.2023.06.001

Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition

WANG Wen-juan¹ DING Yu-tong² SU Hao³ REN Jie⁴ SUN Yan-rong¹ WANG Han-fei¹LU Jia-li⁵ ZHANG Lin-qian⁵ BAI Yu¹ QIN Li-hua^1*

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Abstract

Objective To elucidate the important role of Nogo-A in climacteric neurodegeneration such as memory impairment by observing memory function and the expression of Nogo-A in hippocampus and striatum of rats under low estrogen condition. Methods Fouthy-five female SD rats were divided into sham operation group, ovariectomized group and ovariectomized estrogen treatment group with 15 rats in each group. Medication was given 2 weeks after ovariectomized. Estrogen treatment group was subcutaneously injected in groin with estrogen ［25 μg/(kg·d)］ dissolved in sterile sesame oil. The sham operation group and the ovariectomized group were given the same amount of aseptic sesame oil. Samples were collected after 6 weeks of drug treatment. The difference of memory function of rats in three groups was observed by conditioned fear training experiment, and the expression of Nogo-A in hippocampus and striatum was observed by immunohistochemistry and Western blotting. Results Compared with the sham and estrogen treatment group, memory function in ovariectomized group decreased significantly and the number of Nogo-A positive neurons in hippocampus and striatum of ovariectomized rats was significantly higher than that of sham operation group (P< 0.05). There was no difference between the estrogen treatment group and the sham operation group with the memory function and the Nogo-A expression (P> 0.05). The result of immunoblotting was consistent with the above-mentioned immunohistochemical result. Conclusion The increased expression of Nogo-A in hippocampus and striatum under low estrogen condition may be one of the key reasons for memory impairment in climacteric women.

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Low estrogen / Hippocampus / Striatum / Nogo-A / Memory function / Western blotting / Rat

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WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition[J]. Acta Anatomica Sinica. 2023, 54(6): 620-627 https://doi.org/10.16098/j.issn.0529-1356.2023.06.001

References

［1］Echeverria V, Echeverria F, Barreto GE, et al. Estrogenic plants: to prevent neurodegeneration and memory loss and other symptoms in women after menopause ［J］. Front Pharmacol, 2021, 12: 644103.
［2］Gurvich C, Le J, Thomas N, et al. Sex hormones and cognition in aging ［J］. Vitam Horm, 2021, 115: 511-533.

［3］Zhang N, Cui Y, Li Y, et al. A novel role of nogo proteins: regulating macrophages in inflammatory disease ［J］. Cell Mol Neurobiol, 2022, 42(8):2439-2448.

[4］Seiler S, Di Santo S, Widmer HR. Nogo-A neutralization improves graft function in a rat model of parkinson’s disease ［J］. Front Cell Neurosci, 2016, 10: 87.

［5］Tang H, Xu Y, Liu L, et al. Nogo-A/S1PR2 signaling pathway inactivation decreases microvascular damage and enhances microvascular regeneration in PDMCI mice ［J］. Neuroscience, 2020, 449: 21-34.

［6］Spencer SJ, D’Angelo H, Soch A, et al. High-fat diet and aging interact to produce neuroinflammation and impair hippocampal-and amygdalar-dependent memory ［J］. Neurobiol Aging, 2017, 58: 88-101.
［7］Wang W, Bai W, Cui G, et al. Effects of estradiol valerate and remifemin on norepinephrine signaling in the brain of ovariectomized rats ［J］. Neuroendocrinology, 2015, 101 (2): 120-132.
［8］Cora MC, Kooistra L, Travlos G. Vaginal cytology of the laboratory rat and mouse: review and criteria for the staging of the estrous cycle using stained vaginal smears ［J］. Toxicol Pathol, 2015, 43 (6): 776-793.
［9］Maffei S, Franchini M, Fortunato L, et al. Long-term effects of a combination of isoflavones, agnus castus and magnolia extracts on climacteric symptoms and cardiometabolic risk profile in postmenopausal women ［J］. Gynecol Endocrinol, 2022, 38 (4): 339-344.
［10］Sourouni M, Zangger M, Honermann L, et al. Assessment of the climacteric syndrome: a narrative review ［J］. Arch Gynecol Obstet, 2021, 304 (4): 855-862.
［11］Marchant IC, Chabert S, Martínez-Pinto J, et al. Estrogen, cognitive performance, and functional imaging studies: what are we missing about neuroprotection ［J］? Front Cell Neurosci, 2022, 16: 866122.
［12］Soto A, Nieto-Díaz M, Reigada D, et al. miR-182-5p regulates Nogo-A expression and promotes neurite outgrowth of hippocampal neurons in vitro ［J］. Pharmaceuticals (Basel), 2022, 15(5):529-542.
［13］Shimojo GL, da Silva Dias D, Malfitano C, et al. Combined aerobic and resistance exercise training improve hypertension associated with menopause ［J］. Front Physiol, 2018, 9: 1471.
［14］Cai C, Wang L, Li S, et al. Ras inhibitor lonafarnib rescues structural and functional impairments of synapses of Aβ(1-42) mice via α7nAChR-dependent BDNF upregulation ［J］.J Neurosci, 2022, 42(31):6090-6107.
［15］Cohen HS, Lincoln CM, Pavlik VN, et al. Changes in measures of vestibular and balance function and hippocampus volume in alzheimer’s disease and mild cognitive impairment ［J］. Otol Neurotol, 2022, 43(6):e663-e670.
［16］Delekate A, Zagrebelsky M, Kramer S, et al. NogoA restricts synaptic plasticity in the adult hippocampus on a fast time scale ［J］. Proc Natl Acad Sci USA, 2011, 108 (6): 2569-2574.
［17］Gil V, Nicolas O, Mingorance A, et al. Nogo-A expression in the human hippocampus in normal aging and in Alzheimer disease ［J］. J Neuropathol Exp Neurol, 2006, 65 (5): 433-444.
［18］Wang ZhL, Zhang Y, Wang J. Ｒole of Nogo-A and Nogo-A receptors in Alzheimer’s disease ［J］. Acta Annatomica Sinica, 2018,49(4):549-555. (in Chinese)
王兆伦,张艳,王君. Nogo-A及其受体在阿尔茨海默病中的作用［J］.解剖学报,2018,49(4):549-555.
［19］Kress GJ, Yamawaki N, Wokosin DL, et al. Convergent cortical innervation of striatal projection neurons ［J］. Nat Neurosci, 2013, 16 (6): 665-667.
［20］Marino RA, Gaprielian P, Levy R. Systemic D1-R and D2-R antagonists in Non-human primates differentially impact learning and memory while impairing motivation and motor performance ［J］. Eur J Neurosci, 2022, 56(3):4121-4140.
［21］Ryu H, Lee J, Zaman K, et al. Sp1 and Sp3 are oxidative stress-inducible, antideath transcription factors in cortical neurons ［J］. J Neurosci, 2003, 23 (9): 3597-3606.
［22］Waters EM, Yildirim M, Janssen WG, et al. Estrogen and aging affect the synaptic distribution of estrogen receptor β-immunoreactivity in the CA1 region of female rat hippocampus ［J］. Brain Res, 2011, 1379: 86-97.
［23］Baraka AM, Korish AA, Soliman GA, et al. The possible role of estrogen and selective estrogen receptor modulators in a rat model of Parkinson’s disease ［J］. Life Sci, 2011, 88 (19-20): 879-885.