Effects of hypoxia preconditioning on hematology-related indexes through hypoxia inducible factor-1α/stromal cell-derived factor-1 pathway in rats&nbsp;

SUN Juan; YANG Yi; LIN Tao; MOU Ya-Lin; ZHAO Xiu-Li; ZENG Guo-Xi; ZHANG Yan

doi:10.16098/j.issn.0529-1356.2023.05.002

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (5) : 505-511. DOI: 10.16098/j.issn.0529-1356.2023.05.002

Effects of hypoxia preconditioning on hematology-related indexes through hypoxia inducible factor-1α/stromal cell-derived factor-1 pathway in rats

SUN Juan^1* CHEN Jing-wei¹ YANG Yi¹ LIN Tao¹ MOU Ya-lin² ZHAO Xiu-li¹ ZENG Guo-xi¹ ZHANG Yan^3*
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Abstract

Objective To study the role of hypoxia inducible factor-1α (HIF-1α)/stromal cellderived factor-1 (SDF-1) pathway in high altitude hypoxia preconditioning in rat. Methods Seventy-six adult male SD rats, which through fed in low-pressure oxygen chamber (altitude 5000 m) and Xining (altitude 2260 m) to establish the rat model of hypoxia preconditioning. Rats randomly divided into 6 groups: control group (Ctrl), high altitude hypoxic preconditioning 1 day group (HHP-1d), high altitude hypoxic preconditioning 4 days group (HHP-4d), high altitude hypoxic preconditioning 15 days group (HHP-15d), high altitude hypoxic preconditioning 30 days group (HHP-30d), medium altitude hypoxic preconditioning group (MHP). 7.0T small animal MRI was used to observe the intracranial structure, diameter of basilar artery and cerebral blood flow in the hippocampus and brainstem regions by the sequences of T2 weighted images (T2WI) and arterial spin labeling (ASL) in the groups of Ctrl, HHP-4d, HHP-30d and MHP. In each group, blood routine was tested, the concentrations of HIF-1α, SDF-1 in serum, platelet activating factor (PAF）and P-selectin (SELP) in plasma were detected by the method of ELISA. Results In the hypoxia preconditioning groups, intracranial structure and diameter of basilar artery had no significant difference, while cerebral blood flow in the regions of brainstem and hippocampus increased significantly (P<0.05). Meanwhile, red blood cell and white blood cell increased significantly, while platelet decreased significantly in the groups of hypoxia preconditioning (P<0.05). Red blood cell and platelet in MHP group were closer to Ctrl group. The concentrations of HIF-1α and SDF-1 (except HHP-1d group) increased significantly in hypoxia preconditioning groups (P<0.05).The concentrations of PAF and SELP increased significantly in HHP-1d and HHP-15d groups. The concentration of PAF decreased significantly in the HHP-4d and HHP-30d groups, and SELP decreased significantly in HHP-4d group (P<0.05). Conclusion Hypoxia preconditioning can increase oxygen storage and immune defense capacity, improve brain reserve capacity and play the effect of brain protection through HIF-1α/SDF-1 pathway. The best effect preconditioning was feed at medium altitude (altitude 2260 m) for 30 days.

Key words

Hypoxia inducible factor-1α

/ Stromal cell-derived factor-1 / Hypoxia preconditioning / Routine blood / 7.0 T small animal magnetic resonance imaging / Rat

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SUN Juan CHEN Jing-wei YANG Yi LIN Tao MOU Ya-lin ZHAO Xiu-li ZENG Guo-xi ZHANG Yan.

Effects of hypoxia preconditioning on hematology-related indexes through hypoxia inducible factor-1α/stromal cell-derived factor-1 pathway in rats

[J]. Acta Anatomica Sinica. 2023, 54(5): 505-511 https://doi.org/10.16098/j.issn.0529-1356.2023.05.002

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