Effects of astrocytes on the proliferation of neural stem cells in the hippocampal microenvironment of old and young adults

CHENG Yong-Bo; QU Jia-Hua; DAN Wen; LIU Qian-Qian; QIN Jian-Bing; TIAN Mei-Ling; ZHANG Xin-Hua; SHI Wei

doi:10.16098/j.issn.0529-1356.2023.04.001

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (4) : 375-382. DOI: 10.16098/j.issn.0529-1356.2023.04.001

Effects of astrocytes on the proliferation of neural stem cells in the hippocampal microenvironment of old and young adults

CHENG Yong-bo¹ QU Jia-hua¹ SHAN Wen¹ LIU Qian-qian¹ QIN Jian-bin² TIAN Mei-ling² ZHANG Xin-hua² SHI Wei^1*

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Abstract

Objective To investigate the effect and mechanism of astrocytes on the proliferation of neural stem cells (NSCs) in adult and juvenile hippocampus microenvironment. Methods Hippocampal astrocytes were isolated and cultured from 5 female SD rats at day 1 and week 30 postnatal, respectively; Embryonic hippocampus NSCs was isolated and cultured from 1 SD rat at day 15 of gestation; Conditioned astrocyte culture medium（CM） was collected for NSCs culture; Flow cytometry and CCK-8 were used to detect the proliferation of NSCs cultured in CM. Colony stimulating factor 1 (CSF-1) with differential expression was screened by mass spectrometry after cultured astrocyte CM. Western blotting and ELISA were used to verify the result of mass spectrometry. Immunofluorescence, flow cytometry and CCK-8 were used to detect the proliferation of NSCs treated with different concentrations of CSF-1 recombinant protein （20 μg/L, 100 μg/L, 1 mg/L and 5 mg/L）. Results Compared with the adult group, the CM of hippocampal astrocytes in the young group could promote the proliferation of NSCs(P<0.01); Compared with the conditioned medium of hippocampal astrocytes in the juvenile group, the expression of CSF-1 in the hippocampus of the elder group was significantly up-regulated(P<0.01); At 20 μg/L, CSF-1 promoted the proliferation of NSCs(P<0.01), and 5 mg/L CS-1 inhibited significantly the proliferation of NSCs(P<0.01). Conclusion The secretion of CSF-1 by astrocytes in hippocampal microenvironment can regulate the proliferation of NSCs with the development of the times.

Key words

Colony stimulating factor 1 / Hippocampus / Astrocyte / Neural stem cell / Immunofluorescence / Rat

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CHENG Yong-bo QU Jia-hua SHAN Wen LIU Qian-qian QIN Jian-bin TIAN Mei-ling ZHANG Xin-hua SHI Wei. Effects of astrocytes on the proliferation of neural stem cells in the hippocampal microenvironment of old and young adults[J]. Acta Anatomica Sinica. 2023, 54(4): 375-382 https://doi.org/10.16098/j.issn.0529-1356.2023.04.001

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