Relationship between FokⅠ polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province

WU Zhen; DUAN Qian; YUAN Ming; WANG Xue-Ni; CHEN Wen-Jing; LI Yu-Qin; E Luo-Ji; LI Jian-Hua

doi:10.16098/j.issn.0529-1356.2023.03.009

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (3) : 313-318. DOI: 10.16098/j.issn.0529-1356.2023.03.009

Relationship between FokⅠ polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province

WU Zhen^{1, 2} DUAN Qian¹ YUAN Ming¹ WANG Xue-ni¹ CHEN Wen-jing¹ LI Yu-qin³ E-Luo-Ji³ LI Jian-hua^1*

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Abstract

Objective To investigate the relationship between single nucleotide polymorphism (SNP) FokⅠ（rs2228570/rs10735810）of vitamin D receptor (VDR) gene and hypertensive disorder complicating pregnancy (HDCP) in Han nationality women of Qinghai province. Methods A total of 137 Han nationality HDCP subjects (HDCP group) and 146 Han nationality normal pregnant subjects (control group) were selected from Qinghai province. The FokⅠ polymorphism typing in HCDP group and control group was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The mutation was confirmed by sequencing. SPSS 19.0 statistical software was used to test whether there were significant differences between two groups in general clinical data, genotype and allele frequency distribution. Results The frequency of FF Ff ff genotype of FokⅠ in HDCP group and control group were 51.82%, 37.96%, 10.22% and 34.93%, 43.15%, 21.92% respectively (χ²=11.099, P<0.05, the distribution of genotype was different significantly between the two groups), the frequency of FF genotype in HDCP group (OR=2.004, 95%CI=1.243-3.231) was higher than that in control group; The frequency distribution of alleles F and f of FokⅠ was significantly different between HDCP group and control group (χ²=12.454, P<0.001), F allele frequency in HDCP group (OR=1.253, 95%CI=1.105-1.421) was higher than that in control group. Conclusion The FokⅠ polymorphism of VDR gene is significantly correlated with hypertensive disorder complicating pregnancy of Han nationality in Qinghai. The F allele may be the susceptibility gene of HDCP, the FF genotype may be the susceptibility genotype of HDCP.

Key words

Qinghai province

/ Hypertensive disorder complicating pregnancy / Vitamin-D receptor / Genetic polymorphism / FokⅠ site / Polymerase chain reactionrestriction fragment length polymorphism / Pregnant woman

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WU Zhen DUAN Qian YUAN Ming WANG Xue-ni CHEN Wen-jing LI Yu-qin E-Luo-Ji LI Jian-hua.

Relationship between FokⅠ polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province

[J]. Acta Anatomica Sinica. 2023, 54(3): 313-318 https://doi.org/10.16098/j.issn.0529-1356.2023.03.009

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