Monitoring second polar body exclusion by time-lapse in predicting fertilization and embryo development efficiency of intracytoplasmic sperm injection

XUE Lin-Tao; WANG Shi-Kai; MAO Xian-Bao; LI Zheng-Da; ZHANG Xiao-Hui; WEI Ping-Pin

doi:10.16098/j.issn.0529-1356.2023.02.015

PDF(2335 KB)

Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (2) : 226-230. DOI: 10.16098/j.issn.0529-1356.2023.02.015

Histology,Embryology and Developmental Biology

Monitoring second polar body exclusion by time-lapse in predicting fertilization and embryo development efficiency of intracytoplasmic sperm injection

XUE Lin-tao* WANG Shi-kai MAO Xian-bao LI Zheng-da ZHANG Xiao-hui WEI Pin-pin

Author information +

History +

Abstract

Objective To explore the clinical value of second polar body (Pb2) exclusion monitoring by time-lapse in predicting the fertilization and embryo development efficiency for intracytoplasmic sperm injection (ICSI). Methods A retrospective research was performed on 278 patients treated with ICSI, the clinical data and Time-lapse monitoring embryo culture data were collected and analyzed, to explore the exclusion of Pb2 after ICSI and the relationship between the specific exclusion time and the outcome of fertilization and embryo development. Results The average time of Pb2 exclusion after ICSI was (3.03 ± 1.21) hours; The fertilization rate, 2 pronucleus(PN) fertilization rate and 5 days(D5) blastocyst formation rate in the Pb2 exclusion group were significantly higher than those in the without Pb2 exclusion group (99.95% vs 1.75%, P<0.001; 97.18% vs 0.66%, P<0.001; 60.50% vs 16.67%, P<0.05); The 2PN fertilization rate in Pb2 exclusion time >3-4 hours group was significantly higher than that in 0-2 hours group and >5 hours group (98.80% vs 93.81%, P<0.05; 98.80% vs 95.40%, P<0.05); The exclusion time of Pb2 was significantly correlated with the average number of blastomeres in D3 embryos (P<0.001). The D5 blastocyst formation rate of 3-4 hours group was significantly lower than that of >2-3 hours group (56.23% vs 67.23%, P<0.05), >4-5 hours group was significantly lower than 0-2 hours group and >2-3 hours group (46.61% vs 62.30%, P<0.05; 46.61% vs 67.23%, P<0.05), and D5 blastocyst formation rate of >5 hours group was 7.14%, which were significantly lower than that of the other four groups ( P<0.05). The formation rate of D5 high-quality blastocysts in 3-4 hours group was significantly lower than 0-2 hours group and > 2-3 hours group (9.92% vs 16.39%, P<0.05; 9.92% vs 20.72%, P<0.05), and D5 high-quality blastocysts formation rate in > 4-5 hours group was significantly lower than that in > 2-3 hours group (11.02% vs 20.72%, P<0.05). Conclusion Monitoring Pb2 exclusion by Time-lapse can accurately predict fertilization outcome. The time of Pb2 exclusion is significantly correlated with embryo development potential. It is a valuable morphological index to predict fertilization and embryo development outcome in ICSI.

Key words

Polar body / Fertilization / Embryo development / Time-lapse imaging / Intracytoplasmic sperm injection / Human

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

Lin-Tao XUE Shikai Wang Xianbao Mao Zhengda Li Xiaohui Zhang Pinpin Wei. Monitoring second polar body exclusion by time-lapse in predicting fertilization and embryo development efficiency of intracytoplasmic sperm injection[J]. Acta Anatomica Sinica. 2023, 54(2): 226-230 https://doi.org/10.16098/j.issn.0529-1356.2023.02.015

References

［1］Liu Y, Feenan K, Chapple V, et al. Assessing efficacy of day 3 embryo time-lapse algorithms retrospectively: impacts of dataset type and confounding factors［J］. Hum Fertil, 2019, 22(3):1-9.

［2］Liu Y, Chapple V, Roberts P, et al. Time-lapse videography of human oocytes following intracytoplasmic sperm injection: events up to the first cleavage division［J］. Reprod Biol, 2014, 14(4): 249-256.

［3］Huang JL, Chen ShQ, Wang M, et al. Correlation between follicular fluid metabolic markers and oocyte quality in patients with polycystic ovary syndrome［J］. Acta Anatomica Sinica, 2021, 52(2): 289-294. (in Chinese)

黄剑磊，陈书强，王明，等. 卵泡液代谢标志物与多囊卵巢综合征患者卵母细胞质量的相关性［J］. 解剖学报，2021，52(2)：289-294.

［4］Kirkegaard K, Agerholm IE, Ingerslev HJ. Time-lapse monitoring as a tool for clinical embryo assessment［J］. Hum Reprod, 2012, 27(5):1277-1285.

［5］Coticchi G, Renzini MM, Novara PV, et al. Focused time-lapse analysis reveals novel aspects of human fertilization and suggests new parameters of embryo viability［J］. Hum Reprod, 2018, 33(1): 23-31.

［6］Barberet J, Bruno C, Valot E, et al. Can novel early non-invasive biomarkers of embryo quality be identified with time-lapse imaging to predict live birth［J］? Hum Reprod, 2019,34(8):1439-1449.

［7］Rodríguez MB, Gambini A, Clérico G, et al. Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection［J］. Reprod Fertil Dev, 2019, 31(12):1805-1811.

［8］Yeste M, Jones C, Amdani SN, et al. Oocyte activation deficiency: A role for an oocyte contribution?［J］. Hum Reprod Update, 2015, 22(1):23-47.

［9］Chen C, Kattera S. Rescue ICSI of oocytes that failed to extrude the second polar body 6 h post-insemination in conventional IVF［J］. Hum Reprod, 2003,18(10):2118-2121.

［10］Saunders CM, Larman MG, Parrington J, et al. PLC zeta: a sperm-specific trigger of Ca(2+) oscillations in eggs and embryo development［J］. Development, 2002, 129(15):3533-3544.

［11］Vanden MF, Leybaert L, Nikiforaki D, et al. Diagnostic and prognostic value of calcium oscillatory pattern analysis for patients with ICSI fertilization failure［J］. Hum Reprod, 2013,28(1):87-98.

［12］Liao C, Shen X, Zhang Y, et al. Ratio of the zygote cytoplasm to the paternal genome affects the reprogramming and developmental efficiency of androgenetic embryos［J］. Mol Reprod Dev, 2020, 87(4): 493-502

［13］Setti AS, Braga D, Iaconelli A, et al. Increasing paternal age and ejaculatory abstinence length negatively influence the intracytoplasmic sperm injection outcomes from egg﹕haring donation cycles［J］. Andrology, 2020, 8(3):594-601