Association between hydroxysteroid dehydrogenases gene family single-nucleotide polymorphisms and digit ratio

LI Fan; MA Zhan-Bing; ZHANG Jing; YANG Meng-Yi; WANG Yuan; MA Qian; LU Hong; HUO Zheng-Hao; DANG Jie

doi:10.16098/j.issn.0529-1356.2023.02.012

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (2) : 208-215. DOI: 10.16098/j.issn.0529-1356.2023.02.012

Cell and Molecules Biology

Association between hydroxysteroid dehydrogenases gene family single-nucleotide polymorphisms and digit ratio

LI Fan^1,2 MA Zhan-bing ^1,2 ZHANG Jing ^1,2 YANG Meng-yi ^1,2 WANG Yuan ^1,2 MA Qian ^1,2 LU Hong ^1,2 HUO Zheng-hao^1,2 DANG Jie ^1,2*

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Abstract

Objective investigate the association between 8 single-nucleotide polymorphisms (SNPs) of hydroxysteroid dehydrogenase (HSD) gene family and human digit ratio (2D ∶4D). Methods Randomly selected 808 college students (400 males and 408 females) as subjects, the digit ratio of left and right fingers were measured and calculated using computer image software. Eight SNPs (rs1000283, rs2236903, rs5479, rs56303414, rs676387, rs4445895, rs2066474, rs8190478) in HSD11B and HSD17B gene families were genotyped by multiplex PCR. The association between 2D∶4D and different genotypes was analyzed by One-Way ANOVA. Results Female left hand(L)2D ∶3D, L2D ∶4D, L3D ∶4D, right hand(R)2D ∶4D, R2D ∶5D were significantly higher than male (P<0.05). The genotype frequency of rs2236903 (HSD11B1) was significantly different between male and female (P <0.05). A good linkage disequilibrium was found between rs1000283and rs2236903 of HSD11B1 gene, rs5479 and rs56303414 of HSD11B2 gene, but there was no statistical difference in frequency between male and female students(P>0.05). The genotypes frequency of the 8 SNPs were not significantly associated with digit ratio (2D ∶4D) in both males and females (P >0.05). Conclusion There are significant gender differences in digit ratio in Ningxia Han college students, but there is no correlation between digit ratio and 8 SNPs in HSD11B and HSD17B gene families, suggesting that HSD11B and HSD17B gene families may have nothing to do with the formation of human digit ratio.

Key words

Hydroxysteroid dehydrogenase / Single-nucleotide polymorphism / Digit ratio / Ningxia region / Multiplex PCR / Human

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LI Fan MA Zhan-bing ZHANG Jing YANG Meng-yi WANG Yuan MA Qian LU Hong HUO Zheng-hao DANG Jie. Association between hydroxysteroid dehydrogenases gene family single-nucleotide polymorphisms and digit ratio[J]. Acta Anatomica Sinica. 2023, 54(2): 208-215 https://doi.org/10.16098/j.issn.0529-1356.2023.02.012

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