Expression change and role of myeloma cancer gene mRNA, microRNA-540-3p, circular RNA_04996 in the liver inflammatory reaction during the rat liver regeneration

ZHANG Chun-Bo; WANG Gai-Ping; WANG Zi-Hui; ZANG Xia-Yan; XUE Qi-Jie; LIN Kai-Lin; HAN Lu; WANG Qi-Wen; XU Cun-Shuan

doi:10.16098/j.issn.0529-1356.2023.01.010

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Acta Anatomica Sinica ›› 2023, Vol. 54 ›› Issue (1) : 70-74. DOI: 10.16098/j.issn.0529-1356.2023.01.010

Expression change and role of myeloma cancer gene mRNA, microRNA-540-3p, circular RNA_04996 in the liver inflammatory reaction during the rat liver regeneration

ZHANG Chun-bo^1,2 WANG Gai-ping^1,2 WANG Zi-hui^1,2 ZANG Xia-yan^1,2 XUE Qi-jie^1,2LIN Kai-lin^1,2 HAN Lu^1,2 WANG Qi-wen^1,2* XU Cun-shuan^1,2*

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Abstract

Objective To explore the role pathway and pattern of the transcription factor myeloma cancer gene (MYC) and its mRNA interaction with microRNA(miRNA, miR) and ciccular RNA(circRNAs) at 0 hour and 6 hour in the rat liver regeneration. Methods The rat 2/3 hepatectomy (partial hepatectomy，PH) model was prepared as described by Higgins, the expression changes of mRNA, miRNA and circRNA ［together named as competing endogenous RNAs(ceRNA)］ of remnant liver were detected by the large-scale quantitative detection technology, the interaction network of ceRNA was constructed by Cytoscape 3.2 software, and their correlation in expression and role were analyzed by ceRNA comprehensive analysis. Results It was found that at 0 hour and 6 hours after PH, the ratio value of MYC mRNA showed 0.15±0.03 and 2.36±0.20, miR-540-3p displays 3.00±0.43 and 0.79±0.01, circRNA_04996 showed 1.43±0.43 and 3.14±0.94. At the same time, the four kinds of inflammatory reaction-related genes plasminogen activator urokinase receptor (PLAUR), tumor necrosis factor (TNF), interleukin 1 receptor type 2 (IL1R2), ect, which were prometed in expression by MYC, were down-regulated at 0 hour after PH, but the inflammatory reaction-related genes natriuretic peptide A (NPPA), nuclear receptor subfamily O group B member 2 (NROB2) and peroxisome proliferator activated receptor alpha (PPARA), which were inhibited in expression by MYC, were up-regulated at 0 hour after PH. On the other hand, the three kinds of inflammatory reaction-related genes PLAUR, TNF, IL1R2, ect, which are prometed in expression by MYC, were up-regulated at 6 hours after PH, but the inflammatory reaction-related genes NPPA, NROB2 and PPARA, which were inhibited in expression by MYC, were down-regulated at 6 hours after PH. Conclusion The correlation in expression and role of the miRNA, which are inhibited by circRNAs, MYC, its mRNA is inhibited by miRNAs, and the inflammatory reaction-related genes, which are regulated by MYC, and are helpful for the hepatocyte to be in non-inflammatory reaction state at 0 hour after PH and to be in inflammatory reaction state at 6 hours after PH.

Key words

Liver regeneration

/ Inflammatory reaction of remnant liver / Myeloma cancer gene / Biological high-throughput detection / Competing endogenous RNA comprehensive analysis / Rat

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ZHANG Chun-bo WANG Gai-ping WANG Zi-hui ZANG Xia-yan XUE Qi-jie LIN Kai-lin HAN Lu WANG Qi-wen XU Cun-shuan.

Expression change and role of myeloma cancer gene mRNA, microRNA-540-3p, circular RNA_04996 in the liver inflammatory reaction during the rat liver regeneration

[J]. Acta Anatomica Sinica. 2023, 54(1): 70-74 https://doi.org/10.16098/j.issn.0529-1356.2023.01.010

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