Relationship between the G894T polymorphism of endothelial nitric oxide synthase gene and lipid metabolism among hypertensive disorder complicating pregnancy patients

LI Jing-Yun; LIU Hui-Li

doi:10.16098/j.issn.0529-1356.2022.06.007

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (6) : 737-743. DOI: 10.16098/j.issn.0529-1356.2022.06.007

Relationship between the G894T polymorphism of endothelial nitric oxide synthase gene and lipid metabolism among hypertensive disorder complicating pregnancy patients

LI Jing-yun LU Yun-hua ZHENG Li-xia ZHAO Gang LIU Hui-li*

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Abstract

Objective To investigate the relationship between the G894T polymorphism of the endothelial nitric oxide synthase (eNOS) gene and the lipid metabolism in patients with hypertensive disorder complicating pregnancy (HDCP). Methods The 528 cases of HDCP patients admitted to the Xingtai Third Hospital from January 2016 to January 2020 were selected as the research objects, and 128 normal pregnant women during the same period were selected as the control group. The fasting peripheral venous blood of all study subjects in the early morning was collected, and blood lipid indexes, cystatin C (CysC) and uric acid levels and other biochemical index levels were measured. According to the blood lipid level, it was divided into normal blood lipid group and dyslipidemia group. The dyslipidemia group included 4 subgroups ［hyper triglyceride (TG) blood group (TG≥1.70mmol/L), low high-density lipoprotein cholesterol (HDL-C)］ hyper lipidemia group (HDL-C＜1.04mmol/L), high total cholesterol (TC) group (TC≥5.18mmol/L), mixed hyperlipidemia group (TG≥1.70mmol/L, TC≥ 5.18mmol/L)］. In addition, polymerase chain reaction-restriction endonuclease length fragment polymorphism (PCR-RFLP) was used to perform genotyping analysis on the G894T locus of the eNOS gene, which was divided into TT, GT and GG genotypes. The distribution of gene polymorphisms in people with different blood lipid levels was compared, and Logistic regression was used to analyze the influencing factors of dyslipidemia and the relationship between NOS gene G894T polymorphism and dyslipidemia. Results The expected and observed genotypes of GG, GT, TT, eNOS gene G894T locus in the study group and the control group conformed to the Hardy-Weinberg equilibrium law (χ2=0.651, P=0.722; χ2=1.845, P=0.398), and the GG type gene frequency and G allele frequency of the study group were higher than those of the control group, and the TT type gene frequency and T allele frequency of the study group were lower than those of the control group (P<0.05); homocysteine (Hcy), uric acid, C-reactive protein (CRP).The urine microalbumin to creatinine ratio (UACR) level and body mass index(BMI) were higher than those in the normal blood lipid group, and the rate of taking antihypertensive drugs was lower (P<0.05). Dyslipidemia group eNOS gene (G894T) gene GG gene frequency and G allele were higher than normal blood lipid group, TT gene frequency and T allele were lower than normal blood lipid group (P<0.001). The BMI of patients in the low HDL-C group, hyper TG group and mixed hyperlipidemia group was higher than that of the normal blood lipid group (P<0.05); The Hcy level of the mixed hyperlipidemia group was higher than that of the normal blood lipid group (P<0.05). The TT gene frequency and T allele frequency of the low HDL-C group and the mixed hyperlipidemia group were significantly lower than those of the normal blood lipid group, and the GG gene frequency and G allele frequency were higher than the normal blood lipid group (P<0.05). Logistic regression analysis showed that genotype TT was a protective factor for dyslipidemia in patients with HDCP, and genotype GG, high BMI and high Hcy levels were independent risk factors for dyslipidemia. Conclusion There is a significant correlation between the G894T polymorphism of the eNOS gene and dyslipidemia among patients with hypertension in pregnancy. The genotype TT is a protective factor, and the genotype GG/GT is an independent risk factor; at the same time, BMI and Hcy will also affect dyslipidemia.

Key words

Gene polymorphism / Endothelial nitric oxide synthase gene / Hypertension in pregnancy / Lipid metabolism / Correlation / Polymerase chain reaction-restriction endonuclease length fragment polymorphism / Pregnant woman

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LI Jing-yun LU Yun-hua ZHENG Li-xia ZHAO Gang LIU Hui-li. Relationship between the G894T polymorphism of endothelial nitric oxide synthase gene and lipid metabolism among hypertensive disorder complicating pregnancy patients[J]. Acta Anatomica Sinica. 2022, 53(6): 737-743 https://doi.org/10.16098/j.issn.0529-1356.2022.06.007

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