Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues

FAN Ya-Nan; LI Chao-Hong; JIA Xiang-Lei; JIN Jia-Hao; LIU Yu-Zhen

doi:10.16098/j.issn.0529-1356.2022.06.002

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (6) : 698-704. DOI: 10.16098/j.issn.0529-1356.2022.06.002

Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues

FAN Ya-nan^1,2 LI Chao-hong¹ JIA Xiang-lei¹ JIN Jia-hao¹ LIU Yu-zhen^1*

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Abstract

Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in rat superior cervical ganglion and the effect of chronic intermittent hypoxia (CIH) on BACE1 level. Methods The expression and distribution of BACE1 in superior cervical ganglion were detected by RT-PCR, Western blotting and immunohistochemistry. Totally 16 male SD rats were randomly divided into control group and CIH group, 8 rats in each group. After 2 weeks of modeling, the effect of CIH on BACE1 and peroxisome proliferators activated receptor gamma co-activator 1 alpha (PGC-1α) mRNA level was detected by RT-PCR.

Results BACE1 was expressed in rat superior cervical ganglion, and mainly distributed in satellite glial cells and nerve fibers, but not in blood vessels, neurons and small intesely fluorecent(SIF) cells. CIH down-regulated BACE1 mRNA level, but up-regulated PGC-1α mRNA level (P<0.01). Conclusion BACE1 is located in satellite glial cells and nerve fibers in the superior cervical ganglion of rats. The decreased level of BACE1 may be involved in the regulation of CIH-induced synaptic plasticity of superior cervical ganglion.

Key words

β-site amyloid precursor protein cleaving enzyme 1 / Superior cervical ganglion / Chronic intermittent hypoxia / Western blotting / Rat

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FAN Ya-nan LI Chao-hong JIA Xiang-lei JIN Jia-hao LIU Yu-zhen. Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues[J]. Acta Anatomica Sinica. 2022, 53(6): 698-704 https://doi.org/10.16098/j.issn.0529-1356.2022.06.002

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Funding

National Natural Science Foundation Project;National Natural Science Foundation Project;Youth Foundation Project of the First Affiliated Hospital of Xinxiang Medical College;Research Innovation Support Program for Graduate Students of Xinxiang Medical College