Expression of elastin and collagen in rats with heart failure

WANG Ke-Ke; LUO Huan-Huan; MENG Xiang-Guang; WANG Qian; GUO Zhi-Kun

doi:10.16098/j.issn.0529-1356.2022.05.015

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (5) : 637-643. DOI: 10.16098/j.issn.0529-1356.2022.05.015

Histology,Embryology and Developmental Biology

Expression of elastin and collagen in rats with heart failure

WANG Ke-ke¹ LUO Huan-huan² MENG Xiang-guang¹ WANG Qian¹ GUO Zhi-kun^1,3*

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Abstract

Objective To investigate the expression of elastin and collagen in heart of rats with chronic heart failure and their related proteases. Methods Sprague-Dawley rat model of chronic heart failure was made by intraperitoneal injection of doxorubicin hydrochloride. Examination of cardiac function by echocardiography. ELISA and immunohistochemistry were used to detect brain natriuretic peptide (BNP), and Masson staining was used to detect myocardial fibrosis. The mRNA levels of elastin, Ⅰ/Ⅲ collagen were detected by Real-time PCR and the expression levels of elastin, Ⅰ/Ⅲ collagen, Matrix metalloproteinase (MMP)-2 and MMP-9 were detected by Western blotting. Results Compared with the control group, the heart function of rats in the model group decreased, the BNP content in serum increased, the expression of BNP in myocardial tissue was strongly positive, and the mRNA and protein expression of elastin decreased. Nevertheless, the mRNA and protein expression of Ⅰ/Ⅲ collagen increased. At the same time, both MMP-2 and MMP-9 protein expression levels increased in model rat heart. Conclusion Heart failure leads to decreased expression of elastin, Ⅰ/Ⅲ collagen expression increased, and the MMP-2 and MMP-9 may be involved in the regulation of this process.

Key words

Matrix metalloproteinase / Elastin / Collagen / Heart failure / Real-time PCR / Immunofluorescence / Western blotting / Rat

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WANG Ke-ke LUO Huan-huan MENG Xiang-guang WANG Qian GUO Zhi-kun. Expression of elastin and collagen in rats with heart failure[J]. Acta Anatomica Sinica. 2022, 53(5): 637-643 https://doi.org/10.16098/j.issn.0529-1356.2022.05.015

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