Effect of polycomb group facor 1 on the enrichment of colorectal cancer stem cells and its mechanism

JI Guang-Yu; ZHOU Juan; LI Xin-Yue; HAO Hong-Bo

doi:10.16098/j.issn.0529-1356.2022.05.011

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (5) : 613-619. DOI: 10.16098/j.issn.0529-1356.2022.05.011

Cancer Biology

Effect of polycomb group facor 1 on the enrichment of colorectal cancer stem cells and its mechanism

JI Guang-yu¹ ZHOU Juan¹ LI Xin-yue¹ HAO Hong-bo^2*

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Abstract

Objective To explore the effect of epigenetic regulator polycomb group factor 1(PCGF1) on the enrichment of colorectal cancer stem cells and its mechanism. Methods Meta-analysis was used to predict the expression of PCGF1 in normal and colorectal cancer tissues. The gene expression of PCGF1 in colorectal cancer tumor cells and normal intestinal epithelium cells was detected by Real-time PCR. PCGF1 loss-of-function subclone was generated in HCT116, and stemness was evaluated by spheroid formation. The effects of PCGF1 on stemness markers and the key proteins in Wnt and Notch pathways were detected by Oncomine and GEPIA database, Realtime PCR and Western blotting. Results PCGF1 was markedly upregulated in colorectal cancer cells and tissues. PCGF1 knockdown strongly inhibited tumor sphere formation of HCT116 and stemness markers expression. There was a positive correlation between PCGF1 and the key proteins in Wnt and Notch pathways. PCGF1 knockdown reduced the expression of β-catenin in Wnt signaling pathway. Conclusion PCGF1 affects the enrichment of colorectal cancer stem cells and regulates the expression of key proteins in the signal pathway which is related to colorectal cancer stem cells self-renewal.

Key words

Colorectal cancer / Cancer stem cell / Polycomb group factor 1 / Signaling pathway / Real-time PCR

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JI Guang-yu ZHOU Juan LI Xin-yue HAO Hong-bo. Effect of polycomb group facor 1 on the enrichment of colorectal cancer stem cells and its mechanism[J]. Acta Anatomica Sinica. 2022, 53(5): 613-619 https://doi.org/10.16098/j.issn.0529-1356.2022.05.011

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