Molecular mechanism of microRNA-29a-3p targeting Serpinh1 regulating proliferation and invasion of human gastric cancer cell line BGC823

YAN Xiao-Di; XUE Qiang; WANG Fei-Ran; GU Hong-Mei; TANG Chong; LIU Rui-Qi; ZHOU You-Lang

doi:10.16098/j.issn.0529-1356.2022.05.010

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (5) : 607-612. DOI: 10.16098/j.issn.0529-1356.2022.05.010

Molecular mechanism of microRNA-29a-3p targeting Serpinh1 regulating proliferation and invasion of human gastric cancer cell line BGC823

YAN Xiao-di¹ XUE Qiang¹ WANG Fei-ran¹ GU Hong-mei¹ TANG Chong² LIU Rui-qi³ ZHOU You-lang¹ LIU Xian-cheng^1*

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Abstract

Objective To investigate the effects of microRNA(miR)-29a-3p on the proliferation and invasion of gastric cancer cells and analyze its related molecular mechanism. Methods The expression level of miR-29a-3p in gastric cancer cells was detected, and the role of miR-29a-3p in the proliferation, migration, and invasion of gastric cancer cells was evaluated. Western blotting and luciferase analysis showed that miR-29a-3p was directly bound to Serpinh1 3’-untranslated region(3’UTR). In addition, the effects of the miR-29a-3p/Serpinh1 axis on the proliferation, migration, and invasion of gastric cancer cells were detected by MTT assay, colony formation assay, and Transwell assay in vitro. Results After transfection, the expression of miR-29a-3p in the miR-29a-3p mimic group was significantly higher than that in the miR-29a-3p negative control and blank group. After transfection, the proliferation of BGC823 cells decreased significantly. Luciferase analysis showed that miR-29a-3p inhibited the expression of Serpinh1 by targeting the 3’UTR of Serpinh1. In addition, overexpression of miR-29a-3p significantly inhibited the proliferation, invasion, and migration of gastric cancer cells by targeting Serpinh1. Conclusion MiR-29a-3p can target Serpinh1 and regulate the proliferation and invasion of gastric cancer cells.

Key words

MicroRNA-29a-3p / Serpinh1 / Gastric cancer / Proliferation / Invasion / Western blotting

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YAN Xiao-di XUE Qiang WANG Fei-ran GU Hong-mei TANG Chong LIU Rui-qi ZHOU You-lang LIU Xian-cheng. Molecular mechanism of microRNA-29a-3p targeting Serpinh1 regulating proliferation and invasion of human gastric cancer cell line BGC823[J]. Acta Anatomica Sinica. 2022, 53(5): 607-612 https://doi.org/10.16098/j.issn.0529-1356.2022.05.010

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