Polymorphism of 5,10-methylenetetrahydrofolate reductase gene rs1801131 in Qinghai Han patients with hypertensive disorders complicating pregnancy

YUAN Ming; DUAN Qian; LI Wen-Jia; WANG Ru; WU Zhen; LI Jian-Hua

doi:10.16098/j.issn.0529-1356.2022.05.008

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (5) : 594-599. DOI: 10.16098/j.issn.0529-1356.2022.05.008

Polymorphism of 5,10-methylenetetrahydrofolate reductase gene rs1801131 in Qinghai Han patients with hypertensive disorders complicating pregnancy

YUAN Ming DUAN Qian LI Wen-jia WANG Ru WU Zhen LI Jian-hua*

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Abstract

Objective To investigate the correlation between the polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR) gene rs1801131 and hypertensive disorders complicating pregnancy(HDCP) in Qinghai Han nationality. Methods The polymorphism of MTHFR rs1801131 in 120 pregnant women with HDCP (HDCP group) and 120 normal pregnant women (control group) were detected by restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) and verified by sequencing. Results The frequencies of AA, AC, and CC genotype of MTHFR gene in the HDCP group were 56.67%, 32.50%, and 10.83% respectively, and those in the control group were 74.17%, 23.33% and 2.50% respectively (P<0.05), the distribution of genotype was different significantly between the two groups). The frequency of AA genotype of HDCP group (56.67%) was lower than that of control group (74.17%, P<0.05), the frequency of CC genotype of HDCP group (10.83%) was higher than that of control group (2.50%, P<0.05), while there was no significant difference in the frequency of AC genotype between HDCP group and control group (P<0.05). The frequency distribution of alleles A and C of MTHFR rs1801131 polymorphism was significantly different between the HDCP group and the control group (P<0.001), and the frequency of allele C in the HDCP group was significantly higher than that in the control group (χ2=12.229, OR=1.574, 95% CI=1.181-2.099, P<0.001). Conclusion The polymorphism of MTHFR rs1801131 is related to the occurrence of HDCP in Qinghai Han population. The C gene might be the susceptibility gene of HDCP, and CC genotype might be the susceptibility genotype of HDCP.

Key words

Hypertensive disorders complicating pregnancy / Methylenetetrahydrofolate reductase / Genetic polymorphism / Restriction fragment length polymorphism polymerase chain reaction / Pregnant woman

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YUAN Ming DUAN Qian LI Wen-jia WANG Ru WU Zhen LI Jian-hua. Polymorphism of 5,10-methylenetetrahydrofolate reductase gene rs1801131 in Qinghai Han patients with hypertensive disorders complicating pregnancy[J]. Acta Anatomica Sinica. 2022, 53(5): 594-599 https://doi.org/10.16098/j.issn.0529-1356.2022.05.008

References

［1］Guidelines for Diagnosis and treatment of hypertensive diseases during pregnancy (2020) ［J］. Chinese Journal of Obstetrics and Gynecology, 2020, (4): 227-238. (in Chinese)

妊娠期高血压疾病诊治指南(2020) ［J］. 中华妇产科杂志, 2020, (4): 227-238.

［2］Xing X. Obstetrics and Gynecology ［M］. 9th ed. Beijing: People’s Health Publishing House, 2018: 83-91. (in Chinese)

谢幸. 妇产科学［M］. 第9版. 北京：人民卫生出版社，2018：83-91.

［3］Fang L, Jiabi Q, Senmao Z, et al. Prevalence of hypertensive disorders in pregnancy in China: a systematic review and meta-analysis ［J］. Pregnancy Hypertens，2021, 24: 13-21.

［4］Michita RT, de Lima Kaminski V,Chies JAB. Genetic variants in preeclampsia: lessons from studies in Latin-American populations ［J］. Front Physiol, 2018, 9: 1771.

［5］Coppedè F, Stoccoro A, Tannorella P, et al. Association of Polymorphisms in Genes Involved in One-Carbon Metabolism with MTHFR Methylation Levels ［J］. Int J Mol Sci, 2019, 20(15): 3754.

［6］He ChCh, Guo AH, Wu ML, et al. Bioinformatics analysis of MTHFR C677T和A1298C gene polymorphisms ［J］. Chinese Journal of Birth Health & Heredity, 2021, 29(2):158-161. (in Chinese)

贺婵婵, 郭爱红, 吴曼玲, 等. MTHFR C677T和A1298C基因多态性的生物信息学分析［J］. 中国优生与遗传杂志, 2021, 29(2): 158-161.

［7］Ding ZhY, Chen YY, Deng QX, et al. Association of 5, 10-methylenetetrahydrofolate reductase gene polymorphism and preeclampsia ［J］. Maternal and Child Health Care of China, 2021, 36(16):3828-3831. (in Chinese)

丁忠英, 陈云燕, 邓庆先, 等. 5,10-亚甲基四氢叶酸还原酶基因多态性和子痫前期的相关性［J］. 中国妇幼保健, 2021, 36(16): 3828-3831.

［8］Put N MJvd, Gabre?ls F, Stevens EMB, et al. A second common mutation in the methylenetetrahydrofolate reductase gene: an additional risk factor for neural-tube defects ［J］ ? Am J Hum Genet, 1998, 62(5): 1044-1051.

［9］Mitsumasa U, Gen K. Epidemiology of hypertensive disorders in pregnancy: prevalence, risk factors, predictors and prognosis ［J］. Hypertens Res, 2017, 40(3): 213-220.

［10］Fuchs F, Senat MV, Rey E, et al. Impact of maternal obesity on the incidence of pregnancy complications in France and Canada ［J］. Sci Rep, 2017, 7(1): 10859.

［11］Bilano VL, Ota E, Ganchimeg T, et al. Risk factors of pre-eclampsia/eclampsia and its adverse outcomes in low- and middle-income countries: a WHO secondary analysis ［J］. PLoS One, 2017, 9(3): e91198.

［12］Cheng P, Duan T. China’s new two-child policy: maternity care in the new multiparous era ［J］. BJOG, 2016, 123(Suppl 3): 7-9.

［13］Tomimatsu T, Mimura K, Endo M, et al. Pathophysiology of preeclampsia: an angiogenic imbalance and long-lasting systemic vascular dysfunction ［J］. Hypertens Res, 2017, 40(464): 305-310.

［14］Paolo C, Valeria S, Antonio A, et al. Methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms in breast cancer: a Sardinian preliminary case-control study ［J］. Int J Med Sci, 2019, 16(8): 1089-1095.

［15］Saeedeh S, Mohsen S, Minoo Y, et al. The early-onset preeclampsia is associated with MTHFR and FVL polymorphisms ［J］. Arch Gynecol Obstet, 2015, 291(6): 1303-1312.

［16］Chedraui P, Andrade M E, Salazar-Pousada D, et al. Polymorphisms of the methylenetetrahydrofolate reductase gene (C677T and A1298C) in the placenta of pregnancies complicated with preeclampsia ［J］. Gynecol Endocrinol, 2015, 31(7): 392-396.

［17］Li JP, Lu XZh, Huo Y, et al. Expert consensus on diagnosis and treatment of H-type hypertension ［J］. Chinese Journal of Hypertension, 2016, 24(2):123-127. (in Chinese)

李建平, 卢新政, 霍勇, 等. H型高血压诊断与治疗专家共识［J］. 中华高血压杂志, 2016, 24(2): 123-127.

［18］Yang YL, Yang HL, Shiao SPK. Meta-Prediction of MTHFR Gene Polymorphisms and Air Pollution on the Risk of Hypertensive Disorders in Pregnancy Worldwide ［J］. Int J Environ Res Public health, 2018, 15(2): 326.

［19］Zhang WX. Correlation analysis of homocysteine, folic acid and vitamin B12 with hypertensive disease during pregnancy ［D］. Shijiazhuang: Hebei Medical University, 2020. (in Chinese)

张维雪. 同型半胱氨酸、叶酸及维生素B12与妊娠期高血压疾病的相关性分析［D］. 石家庄：河北医科大学, 2020.

［20］Khidri FF, Waryah YM, Ali FK, et al. MTHFR and F5 genetic variations have association with preeclampsia in Pakistani patients: a case control study ［J］. BMC Med Genet, 2019, 20(1): 163.

［21］Linjing Z, Lili S,Tao W. Correlation between MTHFR gene polymorphism and homocysteine levels for prognosis in patients with pregnancy-induced hypertension ［J］. Am J Transl Res, 2021, 13(7): 8253-8261.

［22］Yuan Z, Xia H, Xuan X, et al. The association between maternal methylenetetrahydrofolate reductase C677T and A1298C polymorphism and birth defects and adverse pregnancy outcomes ［J］. Prenat Diagn, 2019, 39(1): 3-9.

［23］Beth B, Euser AG, Bol KA, et al. High-altitude residence alters blood-pressure course and increases hypertensive disorders of pregnancy ［J］. J Matern Fetal Neonatal Med, 2020,35(7): 1264-1271.