Effect of long non-coding RNA nuclear-enriched abundant transcript on hypoxi-reoxygenation rat astrocyte injury by targeting microRNA-761

CAI Mei-Zhi; MA You-Quan

doi:10.16098/j.issn.0529-1356.2022.05.005

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (5) : 571-577. DOI: 10.16098/j.issn.0529-1356.2022.05.005

Effect of long non-coding RNA nuclear-enriched abundant transcript on hypoxi-reoxygenation rat astrocyte injury by targeting microRNA-761

CAI Mei-zhi^1* LU Bao-quan¹ WU Xiu-ling¹ SHI Jia¹ MA You-quan²

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Abstract

Objective To investigate the effect of long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) on hypoxia-reoxygenation(H/R) glial astrocyte injury, and to explore whether the mechanism was related to the regulation of micro RNA (miR)-761. Methods Rat cortical astrocytes were cultured to construct a H/R injury model. Astrocytes were divided into control group, model group, model+ small interfering RNA negative control (si-NC) group, model+si-NEAT1 group, model+miR-NC group, model+miR-761 group, model+si-NEAT1+anti-miR-NC group, model+si-NEAT1+anti-miR-761 group. Expression of NEAT1 and miR-761 were detected by Real-time PCR. The experiment was repeated 3 times. The content of malonaldehyde (MDA), and the activity of superoxide dismutase (SOD) and catalase (CAT) were detected by kits. Dual luciferase reporter experiment and Real-time PCR were used to analyze the targeting relationship between NEAT1 and miR-761. The experiment was repeated 3 times. Results Compared with the control group, the cell apoptosis rate and MDA content of the model group increased significantly, SOD and CAT activities decreased significantly, NEAT1 expression increased significantly, and miR-761 expression decreased significantly (P<0.05). Compared with the model+si-NC group, the apoptosis rate and MDA content of the model+si-NEAT1 group reduced significantly, and SOD and CAT activities increased significantly (P<0.05). Compared with the model+miR-NC group, the apoptosis rate and MDA content of the model+miR-761 group reduced significantly, and SOD and CAT activities increased significantly (P<0.05). MiR-761 was the target gene of NEAT1, and NEAT1 negatively regulated miR-761 expression. Compared with the model+si-NEAT1+anti-miR-NC group, the apoptosis rate and MDA content of the model+si-NEAT1+anti-miR-761 group increased significantly, and SOD and CAT activities decreased significantly (P<0.05). Conclusion Interference with NEAT1 expression can protect astrocytes from H/R injury by up-regulating miR-761.

Key words

Long non-coding RNA / Nuclear-enriched abundant transcript 1 / Astrocyte / Hypoxia-reoxygenation / MicroRNA-761 / Real-time PCR / Flow cytometry

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CAI Mei-zhi LU Bao-quan WU Xiu-ling SHI Jia MA You-quan. Effect of long non-coding RNA nuclear-enriched abundant transcript on hypoxi-reoxygenation rat astrocyte injury by targeting microRNA-761[J]. Acta Anatomica Sinica. 2022, 53(5): 571-577 https://doi.org/10.16098/j.issn.0529-1356.2022.05.005

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