Protective effect of long non-coding RNA cytoskeleton regulatory RNA targeting microRNA-1246 on cell damage in Parkinson’s disease models

LI Wei; WANG Li; WANG Zhi-Hua; LIU Qing-Chun; HAN Rong-Sheng

doi:10.16098/j.issn.0529-1356.2022.05.004

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (5) : 563-570. DOI: 10.16098/j.issn.0529-1356.2022.05.004

Protective effect of long non-coding RNA cytoskeleton regulatory RNA targeting microRNA-1246 on cell damage in Parkinson’s disease models

LI Wei^1* WANG Li² WANG Zhi-hua³ LIU Qing-chun¹ HAN Rong-sheng⁴

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Abstract

Objective To study the effect of long non-coding RNA (lncRNA) cytoskeleton regulatory RNA (CYTOR) targeting microRNA (miR)-1246 on cell damage in Parkinson’s disease (PD) models. Methods SK-N-SH cells were exposed to 100 μmol/L 1-methyl-4-phenylpyridinium (MPP+) to establish a PD cell model in vitro. The levels of CYTOR and miR-1246 were determined by Real-time PCR. pcDNA-CYTOR or anti-miR-1246, or co-transfect pcDNA-CYTOR and miR-1246 mimics were transfected into SK-N-SH cells, repectively. After treated with MPP+, flow cytometry was used to assess cell apoptosis, and commercial kits was used to monitor malondialdehyde (MDA) content and glutathione peroxidase (GSH) activity. The interaction between CYTOR and miR-1246 was verified by dual-luciferase reporter assay. Results CYTOR reduced (P<0.05), while miR-1246 increased (P<0.05) in PD model cells. CYTOR overexpression or miR-1246 inhibition could reduce the apoptosis rate and MDA content of model cells (P<0.05), and increase GSH activity (P<0.05). MiR-1246 was the target gene of CYTOR, and CYTOR negatively regulated miR-1246 expression. Up-regulation of miR-1246 could reverse the effect of CYTOR overexpression on apoptosis and oxidative damage of model cells (P<0.05). Conclusion LncRNA CYTOR can reduce cell apoptosis and oxidative damage in PD models by targeting miR-1246.

Key words

Long non-coding RNA / Cytoskeleton regulatory RNA / MicroRNA-1246 / Oxidative damage / Flow cytometry / Parkinson’s disease cell model

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LI Wei WANG Li WANG Zhi-hua LIU Qing-chun HAN Rong-sheng. Protective effect of long non-coding RNA cytoskeleton regulatory RNA targeting microRNA-1246 on cell damage in Parkinson’s disease models[J]. Acta Anatomica Sinica. 2022, 53(5): 563-570 https://doi.org/10.16098/j.issn.0529-1356.2022.05.004

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