Effect of draxin knockdown on the projection of 23C10-positive neural fibers in the embryonic chick hindbrain

WANG Zi-Yi; ZHANG Shu-Han; ZHANG Zi-Ren; ZHANG Wen-Wen; HU Ya-Nan; ZHANG San-Bing; SU Yu-Hong

doi:10.16098/j.issn.0529-1356.2022.04.003

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (4) : 418-423. DOI: 10.16098/j.issn.0529-1356.2022.04.003

Effect of draxin knockdown on the projection of 23C10-positive neural fibers in the embryonic chick hindbrain

WANG Zi-yi¹ ZHANG Shu-han² ZHANG Zi-ren² ZHANG Wen-wen² HU Ya-nan² ZHANG San-bing^3* SU Yu-hong^2*

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Abstract

Objective To investigate the effects of the downregulation of draxin expression on the projection characteristics of 23C10-positive neural fibers in the chick embryonic hindbrain. Methods The vitro incubation of HH stages 21-22 chick embryonic hindbrain biopsy with alkaline phosphatase （ALP）protein was used as control group. The incubation of HH stages 21-22 chick embryonic hindbrain biopsy with draxin-ALP fusion protein was used as experimental group. The number of embryonic hindbrain for each group was 10. To detect whether 23C10-positive neural fibers could directly bind to draxin protein or not；In ovo electroporation using empty vector in the chick embryonic hindbrain was used as control group. In ovo electroporation with small interfering RNA(siRNA) expressing vector for reducing draxin expression in the chick embryonic hindbrain was used as experimental group. The number of embryonic hindbrain for each group was 18. The effect of the down-regulation of draxin expression and the change of projection characteristics of 23C10-positive neural fibers were observed to check whether the down-regulation of draxin expression would affect the distribution of 23C10-positive fibers. Results Most portion of draxin protein could overlap with 23C10-positive neural fibers in HH stages 21-22 chick embryonic hindbrain biopsies；After expression of the siRNA plasmid against draxin by electroporation, the expression level of draxin protein was significantly reduced, and the distribution of 23C10-positive fibers was scattered in the dorsal hindbrain on the electroporated side at HH stages 25-26 of chick embryos（P<0.05）. Conclusion Draxin protein may directly bind to 23C10-positive fibers in hindbrain, and it plays an important regulatory role in the fasciculation of 23C10-positive fibers during chick embryonic development.

Key words

Draxin / Axonal projection / Hindbrain / Electroporation / Immunohistochemistry / Chick embryo

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WANG Zi-yi ZHANG Shu-han ZHANG Zi-ren ZHANG Wen-wen HU Ya-nan ZHANG San-bing SU Yu-hong. Effect of draxin knockdown on the projection of 23C10-positive neural fibers in the embryonic chick hindbrain[J]. Acta Anatomica Sinica. 2022, 53(4): 418-423 https://doi.org/10.16098/j.issn.0529-1356.2022.04.003

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