Dicer requiring for astrocyte quiescence in developing brains

LIU Kun; CHEN Jing-Fei; WANG Shou-Yu; LI Tao; GAO Xing; YU Bin; MEI Feng; XIAO Lan

doi:10.16098/j.issn.0529-1356.2022.04.001

PDF(12011 KB)

Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (4) : 407-411. DOI: 10.16098/j.issn.0529-1356.2022.04.001

Dicer requiring for astrocyte quiescence in developing brains

LIU Kun¹ CHEN Jing-fei¹ WANG Shou-yu¹ LI Tao¹ GAO Xing¹ YU Bin² MEI Feng^{1, 2*} XIAO Lan^1,2*

Author information +

History +

Abstract

Objective To explore whether microRNAs(miRNA) in astrocytes participate in regulating the phenotypic switch in the developing central nervous system(CNS). Methods The hGFAP-CreERT;Dicer fl/fl;mT/mG and Dicer fl/fl;mT/mG mice were generated and induced by tamoxifen(TMF). They were divided into 7 days, 10 days, 14 days Dicer conditional knock out (Dicer CKO) group and control group according to their age and the expression of hGFAP-CreERT. These 24 mice were divided into six groups and each group contained four mice. Then the change of astrocytes in the developing CNS was observed by immunofluorescenct staining. Results Both of the densities of astrocytes and microglial cells increased in the Dicer CKO mice brains as compared to littermate controls. Conclusion Dicer and miRNA of astrocytes are important for astrocytes quiescence in developing brains.

Key words

White matte / Astrocyte / microRNA / Microglia / Glial fibrillary acidic protein / Immunofluorescence / Mouse

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LIU Kun CHEN Jing-fei WANG Shou-yu LI Tao GAO Xing YU Bin MEI Feng XIAO Lan. Dicer requiring for astrocyte quiescence in developing brains[J]. Acta Anatomica Sinica. 2022, 53(4): 407-411 https://doi.org/10.16098/j.issn.0529-1356.2022.04.001

References

［1］Sofroniew M, Vinters H. Astrocytes: biology and pathology［J］. Acta Neuropathol, 2010, 119(1): 7-35.

［2］Nash B, Ioannidou K, Barnett S. Astrocyte phenotypes and their relationship to myelination［J］. J Anat, 2011, 219(1): 44-52.

［3］Sloan S, Barres B. Mechanisms of astrocyte development and their contributions to neurodevelopmental disorders［J］. Current Opin Neurobiol, 2014, 27(1): 75-81.

［4］Zou LQ, Jin GH, Li HM, et al. Activated astrocyte secrete clusterin that enhances neuronal differentiation from rat hippocampal neural stem cells［J］.Acta Anatomica Sinica, 2013,44(6):761-766.(in Chinese)

邹琳清,金国华,李浩明,等. 激活的星形胶质细胞分泌聚集素蛋白及其对海马神经干细胞向神经元分化的促进作用［J］.解剖学报,2013,44(6):761-766.

［5］Zhuang WX,Fu WY,Lü E,et al.Chinese of microglia and astrocyte in substantia nigra of 6-OHDA model rats of Parkinson disease［J］.Acta Anatomica Sinica,2010,41(3):344-348.(in Chinese)

庄文欣,付文玉,吕娥,等.帕金森病大鼠黑质小胶质细胞及星形胶质细胞的变化［J］.解剖学报,2010,41(3):344-348.

［6］Varol D, Mildner A, Blank T, et al. Dicer deficiency differentially impacts microglia of the developing and adult brain［J］. Immunity, 2017, 46(6): 1030-1044.

［7］Dugas J, Cuellar T, Scholze A, et al. Dicer1 and miR-219 Are required for normal oligodendrocyte differentiation and myelination［J］. Neuron, 2010, 65(5): 597-611.

［8］Hirrlinger P, Scheller A, Braun C, et al. Temporal control of gene recombination in astrocytes by transgenic expression of the tamoxifen-inducible DNA recombinase variant CreERT2［J］. Glia, 2006, 54(1): 11-20.

［9］Sardo L, Vakil P, Elbezanti W, et al. The inhibition of microRNAs by HIV-1 Tat suppresses beta catenin activity in astrocytes［J］.Retrovirology, 2016, 13(1): 25-34.

［10］ Sun C, Zhu L, Ma R, et al. Astrocytic miR-324-5p is essential for synaptic formation by suppressing the secretion of CCL5 from astrocytes［J］. Cell Death Dis, 2019, 10(2): 141-157.

［11］ Kuang Y, Liu Q, Shu X, et al. Dicer1 and miR-9 are required for proper Notch1 signaling and the Bergmann glial phenotype in the developing mouse cerebellum［J］. Glia, 2012, 60(11): 1734-1746.

［12］ Karimi-Abdolrezaee S, Billakanti R. Reactive astrogliosis after spinal cord injury-beneficial and detrimental effects［J］. Mol Neurobiol, 2012, 46(2): 251-264.

［13］ Tran A, Warren P, Silver J. The biology of regeneration failure and success after spinal cord injury［J］. Physiol Rev, 2018, 98(2): 881-917.

［14］ Mayo L, Trauger S, Blain M, et al. Regulation of astrocyte activation by glycolipids drives chronic CNS inflammation［J］. Nature Med, 2014, 20(10): 1147-1156.

［15］ Molofsky A, Krencik R, Krenick R, et al. Astrocytes and disease: a neurodevelopmental perspective［J］. Genes Dev, 2012, 26(9): 891-907.

［16］ Birch AM. The contribution of astrocytes to Alzheimer’s disease［J］.Biochem Soc Trans, 2014, 42(5): 1316-1320.

［17］ Skripuletz T, Hackstette D, Bauer K, et al. Astrocytes regulate myelin clearance through recruitment of microglia during cuprizone-induced demyelination［J］. Brain, 2013, 136(Pt1): 147-167.

［18］ Nash B, Thomson C, Linington C, et al. Functional duality of astrocytes in myelination［J］. J Neurosci, 2011, 31(37): 13028-13038.