Association between methylenetetrahydrofolate reductase polymorphisms and hypertensive disorders of pregnancy in Han nationality in Qinghai Province

DUAN Qian; LI Jian-Hua; LI Wen-Jia; WANG Ru

doi:10.16098/j.issn.0529-1356.2022.03.013

PDF(1285 KB)

Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (3) : 354-359. DOI: 10.16098/j.issn.0529-1356.2022.03.013

Association between methylenetetrahydrofolate reductase polymorphisms and hypertensive disorders of pregnancy in Han nationality in Qinghai Province

DUAN Qian LI Jian-hua* LI Wen-jia WANG Ru

Author information +

History +

Abstract

Objective To investigate the association between polymorphisms of methylene tetrahydrofolate reductase (MTHFR)-677C/T (rs1801133) and hypertensive disorders of pregnancy (HDP) in Han women of Qinghai Province. Methods A total of 139 HDP subjects (HDP group) and 145 normal pregnant subjects (control group) were selected from Qinghai province. The MTHFR-677C/T polymorphism typing in HDP group and control was analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The mutation was confirmed by sequencing. Results The frequency of CC CT TT genotype of MTHFR gene in HDP group and control group were 54.68%，35.25%，10.07% and 69.66%，22.06%，8.28% respectively (P<0.05);The frequency of CC genotype (54.68%) in HDP group was lower than that(69.66%) in control group (P<0.05),the frequency of CT genotype ( 35.25%) in HDP group was higher than that (22.06%) in control group (P<0.05),there was no significant difference in the frequency of TT genotype between HDP group and control group (P>0.05);The frequency distribution of alleles C and T of MTHFR-677C/T polymorphisms were different between HDP group and control group (P<0.05),T allele frequency in HDP group was higher than that in control group(χ²=5.568，P<0.05). Conclusion The polymorphisms of MTHFR -677C/T is significantly correlated with hypertensive disorders of pregnancy of Han group in Qinghai Province. The T allele of MTHFR -677C/T polymorphism may be the susceptibility gene of HDP,CT genotype is the susceptibility genotype of HDP.

Key words

Hypertensive disorder of pregnancy / Methylenetetrahydrofolate retractase / Genetic polymorphism / Folate metabolism / Restriction fragment length polymorphism polymerase chain reaction / Pregnant woman

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

DUAN Qian LI Jian-hua LI Wen-jia WANG Ru. Association between methylenetetrahydrofolate reductase polymorphisms and hypertensive disorders of pregnancy in Han nationality in Qinghai Province[J]. Acta Anatomica Sinica. 2022, 53(3): 354-359 https://doi.org/10.16098/j.issn.0529-1356.2022.03.013

References

［1］Brown MA, Magee LA, Kenny LC, et al. Hypertensive disorders of pregnancy: ISSHP classification, diagnosis, and management recommendations for international practice ［J］. Hypertension, 2018, 72(1): 24-43.

［2］Umesawa M, Kobashi G. Epidemiology of hypertensive disorders in pregnancy: prevalence, risk factors, predictors and prognosis ［J］. Hypertens Res, 2017, 40(3): 213-220.

［3］Li F, Qin J, Zhang S, et al. Prevalence of hypertensive disorders in pregnancy in China: a systematic review and meta-analysis ［J］. Pregnancy Hypertens, 2021, 24(2):13-21.

［4］Al Mutairi F. Hyperhomocysteinemia: clinical insights ［J］. J Cent Nerv Syst Dis, 2020, 12:1179573520962230.

［5］Sultana R, Choudhury SS, Bose S, et al. Increased homocysteine expression associated with genetic changes in the folate pathway as a key determinant of preeclampsia: A prospective study from lower Assam, India ［J］. Meta Gene, 2020, 26:100775.

［6］Wan WD, Wang LJ, Zhang QG, et al. Study on the relationship between C677T and A1298C polymorphism of MTHFR gene and non-synchromatic cleft lip and palate in some Chinese population［J］. Chinese Journal of Plastic Surgery, 2006, 22(1):8-11.(in Chinese)

万伟东, 王丽君, 章庆国, 等. MTHFR 基因 C677T 和 A1298C 多态与中国部分人群非综合征性唇腭裂的相关研究［J］.中华整形外科杂志, 2006, 22(1):8-11.

［7］Cornelius DC. Preeclampsia: from inflammation to immunoregulation ［J］. Clin Med Insights Blood Disord, 2018, 11:1179545X17752325.

［8］Sato Y. Endovascular trophoblast and spiral artery remodeling ［J］. Mol Cell Endocrinol, 2020, 503:110699.

［9］Shah DA, Khalil RA. Bioactive factors in uteroplacental and systemic circulation link placental ischemia to generalized vascular dysfunction in hypertensive pregnancy and preeclampsia ［J］. Biochem Pharmacol, 2015, 95(4): 211-226.

［10］Phipps E, Prasanna D, Brima W, et al. Preeclampsia: updates in pathogenesis, definitions, and guidelines ［J］. Clin J Am Soc Nephrol, 2016, 11(6): 1102-1113.

［11］Fuchs F, Senat MV, Rey E, et al. Impact of maternal obesity on the incidence of pregnancy complications in France and Canada ［J］. Sci Rep, 2017, 7(1): 1-9.

［12］Pavan LT, Hermouet A, Tsatsaris V, et al. Lipids from oxidized low-density lipoprotein modulate human trophoblast invasion: involvement of nuclear liver X receptors ［J］. Endocrinology, 2004, 145(10): 4583-4591.

［13］Abassi Z, Kinaneh S, Skarzinski G, et al. Aberrant corin and PCSK6 in placentas of the maternal hyperinsulinemia IUGR rat model ［J］. Pregnancy Hypertens, 2020, 21(5): 70-76.

［14］Yakub M, Moti N, Parveen S, et al. Polymorphisms in MTHFR, MS and CBS genes and homocysteine levels in a Pakistani population ［J］. PLoS One, 2012, 7(3): e33222.

［15］Kwak-Kim J, Mizunuma H, Damvaeba S, et al. Effects of MTHFR C677T polymorphism on vitamin D, homocysteine and natural killer cell cytotoxicity in women with recurrent pregnancy losses ［J］. Hum Reprod, 2020, 35(6): 1276-1287.

［16］Mohanraj PS, Rahat B, Mahajan A, et al. Temporal expression of genes involved in folate metabolism and transport during placental development, preeclampsia and neural tube defects ［J］. Mol Biol Rep, 2019, 46(3): 3193-3201.

［17］Shen X, Huang Y, Tang S, et al. The research on the relationshipbetween the polymorphism of MTHFR gene and HCY in patients with hypertensive disorder complication pregnancy ［J］. Chin J Birth Health Heredity, 2008, 16(8): 17-19.

［18］Ahmed SF, Ali MM, Kheiri S, et al. Association of methylenetetrahydrofolate reductase C677T and reduced-f carrier-1 G80A gene polymorphism with preeclampsia in Sudanese women ［J］. Hypertens Pregnancy, 2020, 39(2): 77-81.

［19］Khidri FF, Waryah YM, Ali FK, et al. MTHFR and F5 genetic variations have association with preeclampsia in Pakistani patients: a case control study ［J］. BMC Med Genomics, 2019, 20(1):1-12.

［20］Vazquez-Alaniz F, Lumbreras-Márquez MI, Sandoval-Carrillo AA, et al. Association of COMT G675A and MTHFR C677T polymorphisms with hypertensive disorders of pregnancy in Mexican mestizo population ［J］. Pregnancy Hypertens, 2014, 4(1): 59-64.

［21］Hou J, Lin L, Guo W, et al. Association of methylenetetrahydrofolate reductase C677T polymorphism with the pre-eclampsia risk in Hakka pregnant women in Southern China ［J］. Gynecol Endocrinol, 2019, 36(4): 322-326.

［22］Bailey B, Euser AG, Bol KA, et al. High-altitude residence alters blood-pressure course and increases hypertensive disorders of pregnancy ［J］. J Matern Fetal Neonatal Med, 2020, 35(7): 1264-1271.