Expression and significance of fork head box protein N2 and F2 in gliomas

ZHANG Jia; LUO Xiao-Xiao; GUO Xin-Ran; WU Feng; ZHENG Lan-Rong

doi:10.16098/j.issn.0529-1356.2022.02.004

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (2) : 160-165. DOI: 10.16098/j.issn.0529-1356.2022.02.004

Expression and significance of fork head box protein N2 and F2 in gliomas

ZHANG Jia^1,2 LUO Xiao-xiao^1,2 GUO Xin-ran^1,2 WU Feng³ ZHENG Lan-rong^1,2,4*

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Abstract

Objective To observe the expression levels of fork head box protein N2 (FOXN2) and fork head box protein F2 (FOXF2) in normal brain tissue, low-grade glioma and high-grade glioma, and to explore the relationship between the two and the grade of glioma, and then deduce their roles in the occurrence and development of glioma, to look for possibilities target of drug therapy. Methods From January 2016 to December 2020, 36 glioma specimens were collected from the Department of Pathology, Yijishan Hospital, Wannan Medical College. The expressions of FOXN2 and FOXF2 were detected by immunohistochemistry and immunofluorescence with the double-blind method , and analyzed by SPSS18.0 software, with a statistical significance of P<0.05. Then Western blotting and Real-time PCR experiments were carried out on fresh normal brain tissues, low-grade glioma tissues, high-grade glioma tissues during neurosurgery in Yijishan Hospital of Wannan Medical College from January 2020 to January 2021 (normal brain tissues were all trauma patients). Results The results of immunohistochemistry and immunofluorescence showed that the higher grade of glioma, the lower expressions of FOXN2 and FOXF2 would be (P<0.05). Western blotting showed that the expressions of FOXN2 and FOXF2 in normal brain tissue and low-grade glioma were higher than those in high-grade glioma (P<0.05). The result of Real-time PCR showed that the expressions of FOXN2 and FOXF2 in normal brain tissue were higher than those in high-grade glioma (P<0.01). Conclusion FOXN2 and FOXF2 are expressed in normal brain tissues, and their expression is low in glioma in a grade-dependent manner, suggesting that FOXN2 and FOXF2 are related to the grade and poor prognosis of glioma. Enhancing the expression of FOXN2 and FOXF2 may provide a new idea for target therapy of glioma.

Key words

Fork head box protein N2 / Fork head box protein F2 / Glioma / Immunohistochemistry / Immunofluorescence / Western blotting / Real-time PCR / Human

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ZHANG Jia LUO Xiao-xiao GUO Xin-ran WU Feng ZHENG Lan-rong. Expression and significance of fork head box protein N2 and F2 in gliomas[J]. Acta Anatomica Sinica. 2022, 53(2): 160-165 https://doi.org/10.16098/j.issn.0529-1356.2022.02.004

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