Dihydromyricetin down-regulating the expression of nod-like receptor protein-3 in the brain tissue of ischemic stroke in rats

DING Hui-Ru; DENG Xin-Wei; LIU Huai-Cun; CHENG Quan-Cheng; FANG Xuan; ZHANG Guo-Liang; CHEN Chun-Hua; ZHANG Wei-Guang

doi:10.16098/j.issn.0529-1356.2022.02.001

PDF(7112 KB)

Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (2) : 137-143. DOI: 10.16098/j.issn.0529-1356.2022.02.001

Dihydromyricetin down-regulating the expression of nod-like receptor protein-3 in the brain tissue of ischemic stroke in rats

DING Hui-ru¹ DENG Xin-wei¹ LIU Huai-cun¹ CHENG Quan-cheng¹ FANG Xuan¹ ZHANG Guo-liang² CHEN Chun-hua¹ ZHANG Wei-guang^1*

Author information +

History +

Abstract

Objective To investigate the role of dihydromyricetin (DHM) in the treatment of ischemic stroke in rats, and to explore the effect of DHM on the expression of inflammasome. Methods The middle cerebral artery occlusion (MCAO) model was induced by endovascular suture method. The therapeutic effect and mechanism of DHM were investigated by Longa score, TTC staining, Nissl staining, immunohistochemical staining and Western bloting. Results After DHM treatment, the motor capacity of MCAO rats was significantly improved, the infarct volume was significantly reduced, the brain structure and neuron morphology were improved, and the expressions of nod-like receptor protein-3 (NLRP3) and interleukin-1β(IL-1β) decreased significantly. Conclusion DHM can down-regulate the expression of NLRP3 and thus reduces the cerebral infarction volume and improves neurobehavioral performance in MCAO rats.

Key words

Ischemic stroke / Middle cerebral artery occlusion / Dihydromyricetin / Inflammasome / Nod-like receptor protein-3 / TTC staining / Longa score / Rat

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

DING Hui-ru DENG Xin-wei LIU Huai-cun CHENG Quan-cheng FANG Xuan ZHANG Guo-liang CHEN Chun-hua ZHANG Wei-guang. Dihydromyricetin down-regulating the expression of nod-like receptor protein-3 in the brain tissue of ischemic stroke in rats[J]. Acta Anatomica Sinica. 2022, 53(2): 137-143 https://doi.org/10.16098/j.issn.0529-1356.2022.02.001

References

［1］ Seners P, Turc G, Oppenheim C, et al. Incidence, causes and predictors of neurological deterioration occurring within 24 hours following acute ischaemic stroke: a systematic review with pathophysiological implications［J］. J Neurol Neurosurg Psychiatry, 2015, 86(1):87-94.

［2］ Liu X, Zhang Z, Ruan J, et al. Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores［J］. Nature,2016, 535(7610):153-158.

［3］ Sun P, Yin JB, Liu LH, et al. Protective role of dihydromyricetin in Alzheimer’s disease rat model associated with activating AMPK/SIRT1 signaling pathway［J］. Biosci Rep, 2019, 39(1): BSR20180902.

［4］ Semcheddine F, Guissi NEI, Liu XY, et al. Effects of the preparation method on the formation of true nimodipine SBE-β-CD/HP-β-CD inclusion complexes and their dissolution rates enhancement［J］. AAPS Pharm Sci Tech, 2015, 16(3):704-715.

［5］ Wang J, Jiang C, Li X, et al. The protective mechanism of progesterone on blood-brain barrier in cerebral ischemia in rats［J］. Brain Res Bull, 2009, 79(6):426-430.

［6］ Poh L, Kang SW, Baik SH, et al. Evidence that NLRC4 inflammasome mediates apoptotic and pyroptotic microglial death following ischemic stroke［J］. Brain Behav Immun, 2019, 75:34-47.

［7］ Walsh JG, Muruve DA, Power C. Inflammasomes in the CNS［J］. Nat Rev Neurosci, 2014, 15(2):84-97.

［8］ Fann DYW, Lee SY, Manzanero S, et al. Intravenous immunoglobulin suppresses NLRP1 and NLRP3 inflammasome-mediated neuronal death in ischemic stroke［J］. Cell Death Dis,2013, 4(9):e790.

［9］ Diaz-Ca?estro C, Reiner MF, Bonetti NR, et al. AP-1 (activated protein-1) transcription factor Jun D regulates ischemia/reperfusion brain damage via IL-1β (interleukin-1β) ［J］. Stroke, 2019, 50(2):469-477.

［10］ Shen Y, Lindemeyer AK, Gonzalez C, et al. Dihydromyricetin as a novel anti-alcohol intoxication medication［J］. J Neurosci, 2012, 32(1):390-401.

［11］ Hu Q, Zhang T, Yi L, et al. Dihydromyricetin inhibits NLRP3 inflammasome-dependent pyroptosis by activating the Nrf2 signaling pathway in vascular endothelial cells［J］. Biofactors, 2018, 44(2):123-136.

［12］ Feng J, Wang JX, Du YH, et al. Dihydromyricetin inhibits microglial activation and neuroinflammation by suppressing NLRP3 inflammasome activation in APP/PS1 transgenic mice ［J］. CNS Neurosci Ther,2018, 24(12):1207-1218.

［13］ Wang YC, Liu QX, Zheng Q, et al. Dihydromyricetin alleviates sepsis-induced acute lung injury through inhibiting NLRP3 inflammasome-dependent pyroptosis in mice model［J］. Inflammation, 2019, 42(4):1301-1310.

［14］ Wu YJ, Chen ChH. Progress of adjuvant treatment with tissue plasminogen activator beyond time window in ischemic stroke［J］.Acta Anatomica Sinica, 2019, 50(6):850-856. (in Chinese)

吴冶君, 陈春花. 缺血性脑卒中治疗时间窗后辅助溶栓治疗的进展［J］. 解剖学报, 2019, 50(6):850-856.