18α-glycyrrhetinic acid promoting neural stem cells proliferation&nbsp; through antioxidant in the subventricular zone of adult mice

LIU Xue-Qin; NIU Xiao-Jie; WANG Bi-Hui; WANG Ting-Yu; LU Li

doi:10.16098/j.issn.0529-1356.2022.01.002

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Acta Anatomica Sinica ›› 2022, Vol. 53 ›› Issue (1) : 11-18. DOI: 10.16098/j.issn.0529-1356.2022.01.002

18α-glycyrrhetinic acid promoting neural stem cells proliferation through antioxidant in the subventricular zone of adult mice

LIU Xue-qin¹ NIU Xiao-jie¹ WANG Bi-hui¹ WANG Ting-yu2 YANG Jing¹ LU Li^1*

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Abstract

Objective To explore the effect of 18α-glycyrrhetinic acid (18α-GA) on the proliferation of adult mice neural stem cells (NSCs) and its underlying mechanism. Methods One hundred 6-month BALB/c mice were randomly divided into DMSO control group and 18α-GA group (mice were intraperitoneally injected with 40 mg / kg 18α-GA every day for 2 months). The proliferation capability, oxidative status and nuclear factor E2-related factor 2 (Nrf2) level of NSCs in the adult mice subventricular zone (SVZ) were measured through both in vivoand in vitro experiments, including Ki-67 staining, neurosphere formation assay, BrdU incorporation, CCK-8 assay, reactive oxygen species (ROS) detection, superoxide dismutase 1 (SOD1) determination, Real-time PCR and Western blotting. Results Elevated Ki-67 positive cells were observed in SVZ of mice with 18α-GA application. Meanwhile, ROS level attenuated but SOD1 mRNA and protein level increased significantly in the SVZ of 18α-GA group mice, the latter of which were (3.17±0.073) and (2.12±0.02) times respectively than that of the control group (P<0.05 and P<0.001). Likewise, the similar changes were exhibited in vitro data. NSCs of 18α-GA group mice displayed higher proliferation potency confirmed by accelerated neurosphere formation and increased neurosphere number (P<0.001), as well as higher BrdU positive ratio (P<0.01) and NSCs vitality (P<0.001). NSCs of mice with 18α-GA injection exhibited decreased ROS level by 18.91%±4.33% (P<0.05) and enhanced SOD1 level, compared with those in NSCs of DMSO group mice. Furtherly, the Nrf2 expression in SVZ and NSCs of 18α-GA group mice was higher than that of the control group. Conclution 18α-GA administration plays a vital role in the maintainence and amelioration of adult mice NSCs proliferation through activating SOD1 and diminishing ROS aggregations.

Key words

18α-glycyrrhetinic acid / Neural stem cell / Antioxidant / Western blotting / Mouse

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LIU Xue-qin NIU Xiao-jie WANG Bi-hui WANG Ting-yu YANG Jing LU Li. 18α-glycyrrhetinic acid promoting neural stem cells proliferation through antioxidant in the subventricular zone of adult mice[J]. Acta Anatomica Sinica. 2022, 53(1): 11-18 https://doi.org/10.16098/j.issn.0529-1356.2022.01.002

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