Expression and role of CCAAT enhancer binding&nbsp; protein ζ mRNA, microRNA-136-3p and four kinds of circular RNAs of hepatocytes during the rat liver regeneration initiation

XU Cun-Shuan

doi:10.16098/j.issn.0529-1356.2021.06.013

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (6) : 921-624. DOI: 10.16098/j.issn.0529-1356.2021.06.013

Liver regeneration and cell cycle control

Expression and role of CCAAT enhancer binding protein ζ mRNA, microRNA-136-3p and four kinds of circular RNAs of hepatocytes during the rat liver regeneration initiation

GAO Han^1,2 WANG Zi-hui^1,2 ZANG Xia-yan^1,2 JIN Wei^1,2 CHANG Cui-fang^1,2 GUO Jian-lin^1,2* XU Cun-shuan^1,2*

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Abstract

Objective To explore the pathways and patterns which the CCAAT enhancer binding protein ζ (CEBPζ) mRNA, miR-136-3p, rno-Crebrf_0009, rno-Slc38a9_0001, rno-LOC100362999_0001 and rno-Got1_0001 regulate the hepatocytes in G0 phase and G1 phase during rat liver regeneration (LR). Methods The rat 2/3 partial hepatectomy (PH) model was prepared as described by Higgins, the hepatocytes of rat liver right lobes were isolated in 9∶00-11∶00 am according to the method of Smedsrod et al, the large-scale quantitative detection of competitive endogenous RNA(ceRNAs) was processed by the high-throughput biotechnology, the interaction network of ceRNA was constructed by Cytoscape 3.2 software, and the correlation in expression and role of ceRNA was analyzed by ceRNA comprehensive analysis. Results It was found that at 0 hour and 6 hours after PH, the ratio value of CEBPζ mRNA shows 0.97±0.15 and 2.56±0.12, miR-136-3p displays 1.05±0.32 and 0.38±0.04, rno-Got1_0001 indicates 0.33±0.03 and 4.35±0.78, rno-Crebrf_0009 shows 1.17±0.32 and 2.99±0.28, rno-Slc38a9_0001 indicates 0.67±0.08 and 2.64±0.29, rno-LOC100362999_0001 shows 0.25±0.02 and 0.92±0.22. The G0 phase-related genes inhibited by CEBPζ were following, the cyclin dependent kinase 2 associated protein 2 (CDK2AP2) 2.55±0.42 and 0.74±0.11, the target of myb1 membrane trafficking protein (TOM1) 2.80±0.91 and 1.40±0.36, the valosin containing protein (VCP) 2.63±0.17 and 1.10±0.10. The G1 phase-related genes prometed by CEBPζ were following, the cAMP responsive element binding protein 3 like 4 (CREB3L4) 1.13±0.63 and 2.00±0.81, the thioredoxin interacting protein (TXNIP) 1.03±0.07 and 2.50±0.19. Conclusion CEBPζ mRNA is not up-regulated at 0 hour after PH, that is helpful for the expression of the G0 phase-related genes inhibited by CEBPζ and for the hepatocytes to be in G0 phase. On the contrary, the interaction of miR-136-3p and rno-Got1_0001, rno-Crebrf_0009, rno-Slc38a9_0001 and rno-LOC100362999_0001 leads to CEBPζ mRNA to bind with miR-136-3p, to CEBPζ being formed probablely, and to the expression of the G1 phase-related genes promoted by CEBPζ probablely, and to the hepatocytes being in G1 phase at 6 hours after PH.

Key words

Liver regeneration / Hepatocyte in G0 phase Hepatocyte in G1 phase / / CCAAT enhancer binding protein ζ / Competitive endogenous RNA comprehensive aalysis / Biological high-throughput detection / Competitive endogenous RNA comprehensive analysis / Rat

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GAO Han WANG Zi-hui ZANG Xia-yan JIN Wei CHANG Cui-fang GUO Jian-lin XU Cun-shuan. Expression and role of CCAAT enhancer binding protein ζ mRNA, microRNA-136-3p and four kinds of circular RNAs of hepatocytes during the rat liver regeneration initiation[J]. Acta Anatomica Sinica. 2021, 52(6): 921-624 https://doi.org/10.16098/j.issn.0529-1356.2021.06.013

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