Expression and role of CCAAT enhancer binding protein α mRNA, microRNA-144-3p and three kinds of circular RNAs of hepatocytes during the rat liver regeneration termination

XU Cun-Shuan

doi:10.16098/j.issn.0529-1356.2021.06.010

Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (6) : 909-912. DOI: 10.16098/j.issn.0529-1356.2021.06.010

Liver regeneration and cell cycle control

Expression and role of CCAAT enhancer binding protein α mRNA, microRNA-144-3p and three kinds of circular RNAs of hepatocytes during the rat liver regeneration termination

ZANG Xia-yan^{1, 2} WANG Zi-hui^{1, 2} GAO Han^{1, 2}GUO Jian-lin^{1, 2} JIN Wei^1,2 CHANG Cui-fang^{1, 2} XU Cun-shuan^{1, 2*}

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Abstract

Objective To explore the pathways and patterns which CCAAT enhancer binding proteinα(CEBPα) mRNA, miR-144-3p, rno-LOC100365958_0001, rno-Usp3_0010 and rno-AC241873_0010 regulate the hepatocytes to enter G1 phase during rat liver regeneration (LR). Methods The rat 2/3 hepatectomy (partial hepatectomy, PH) model was prepared as described by Higgins, the hepatocytes of rat liver right lobes were isolated in 9∶00-11∶00 am according to the method of Smedsrod et al, the large-scale quantitative detection of ceRNAs was processed by the high-throughput biotechnology, the interaction network of competitive endogenous RNAs(ceRNAs) was constructed by Cytoscape 3.2 software, and the correlation in expression and role of ceRNAs was analyzed by ceRNA comprehensive analysis. Results It was found that at 6 hours and 72 hours after PH, the ratio value of CEBPα mRNA shows 1.12±0.27 and 2.52±0.15, miR-144-3p displays 2.97±0.81 and 0.99±0.36, rno-LOC100365958_0001 indicates 0.16±0.05 and 6.78±0.62, rno-Usp3_0010 shows 0.13±0.01 and 7.61±0.69, rno-AC241873_0010 indicates 0.09±0.02 and 7.53±0.69. The G1 phase-related gene cylin G2(CCNG2) promoted by CEBPα 0.25±0.06 and 1.10±0.22, but the G1 phase-related genes inbibited by CEBPα were following, ETS variant transcription factor 6(ETV6) 2.22±0.68 and 0.99±0.27, MAPK14 2.01±0.32 and 0.85±0.11, the signal transducer and activator of transcription 3(STAT3)为2.88±0.24 and 1.08±0.07, tumor necrosis factor(TNF) 2.29±0.51 and 0.86±0.51. The G0 phase-related genes promoted by CEBPα were following, G0/G1 witch 2(G0S2) 0.64±0.08 and 4.71±0.91, phospholipase A2 group IVA(PLA2G4A) 0.42±0.13 and 2.83±0.62. Conclusion The interaction of miR-144-3p and rno-LOC100365958_0001, rno-Usp3_0010 and rno-AC241873_0010 leads to CEBPα mRNA to bind with miR-144-3p, to CEBPα being formed difficultly, and to the expression of the G1 phase-related genes inbibited by CEBPα probablely, and to the hepatocytes being in G1 phase at 6 hours after PH. On the contrary, CEBPα mRNA is up-regulated at 72 hours after PH, that is helpful for the expression of the G0 phase-related genes promoted by CEBPα and for the hepatocytes to be in G0 phase.

Key words

Partial hepatectomy / Liver regeneration / Hepatocyte in G0 phase / Hepatocyte in G1 phase / CCAAT/enhancer binding protein α Competitive endogenous RNA comprehensive analysis / Biological high-throughput detection / Rat

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ZANG Xia-yan WANG Zi-hui GAO Han GUO Jian-lin JIN Wei CHANG Cui-fang XU Cun-shuan. Expression and role of CCAAT enhancer binding protein α mRNA, microRNA-144-3p and three kinds of circular RNAs of hepatocytes during the rat liver regeneration termination[J]. Acta Anatomica Sinica. 2021, 52(6): 909-912 https://doi.org/10.16098/j.issn.0529-1356.2021.06.010

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