Expression and role of CCAAT enhancer binding protein α mRNA, microRNA-144-3p and three kinds of circular RNAs of&nbsp; hepatocytes during the rat liver regeneration initiation

XU Cun-Shuan

doi:10.16098/j.issn.0529-1356.2021.06.009

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (6) : 901-908. DOI: 10.16098/j.issn.0529-1356.2021.06.009

Liver regeneration and cell cycle control

Expression and role of CCAAT enhancer binding protein α mRNA, microRNA-144-3p and three kinds of circular RNAs of hepatocytes during the rat liver regeneration initiation

ZANG Xia-yan^{1, 2} WANG Zi-hui^{1, 2} LI Ya-fei^{1, 2} GUO Jian-lin^{1, 2} JIN Wei^{1, 2}CHANG Cui-fang³ XU Cun-shuan^{1, 2*}

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Abstract

Objective To explore the pathways and patterns which CCAAT enhancer binding proteinα(CEBPα) mRNA, miR-144-3p, rno-LOC100365958_0001, rno-Usp3_0010 and rno-AC241873_0010 regulate the hepatocytes in G0 phase and G1 phase during rat liver regeneration (LR). Methods The rat 2/3 hepatectomy (partial hepatectomy, PH) model was prepared as described by Higgins, the hepatocytes of rat liver right lobes were isolated in 9∶00-11∶00 am according to the method of Smedsrod, the large-scale quantitative detection of competitive endogenous RNAs(ceRNAs) was processed by the high-throughput biotechnology, the interaction network of ceRNAs was constructed by Cytoscape 3.2 software, and the correlation in expression and role of ceRNAs was analyzed by ceRNA comprehensive analysis. Results It was found that at 0 hour and 6 hours after PH, the ratio value of CEBPα mRNA showed 4.88±0.78 and 1.12±0.27, miR-144-3p displays 1.30±0.11 and 2.97±0.81, rno-LOC100365958_0001 indicateed 6.99±0.55 and 0.16±0.05, rno-Usp3_0010 shows 8.48±0.67 and 0.13±0.01, rno-AC241873_0010 indicates 11.85±0.93 and 0.09±0.02. The G0 phase-related genes promoted by CEBPα were following, cyclin dependent kinase 2 associated protein 2(CDK2AP2) 2.55±0.42 and 0.74±0.11, G0/G1 swich 2(G0S2) 3.85±0.23 and 0.64±0.08, the signal transducer and activator of transcription 1(STAT1) 2.57±0.13 and 1.32±0.13, transforming growth factor beta receptor 2(TGFBR2) 2.77±0.20 and 0.79±0.13, but the G0 phase-related gene cyclin dependent kinase inhibitor 1a(CDKN1a) inbibited by CEBPα 0.39±0.07 and 0.93±0.15. The G1 phase-related gene cyclin G2(CCNG2) promoted by CEBPα 1.19±0.09 and 0.25±0.06, but the G1 phase-related genes inbibited by CEBPα were following, ETS variant transcription factor 6(ETV6) 0.77±0.05 and 2.22±.68, hemoglobin oygenase 1(HMOX1) 1.05±0.21 and 4.57±0.88, MAPK14 1.01±0.15 and 2.01±0.32, STAT3 0.74±0.15 and 2.88±0.24, tumor necrosis factor(TNF) 0.80±0.14 and 2.29±0.51. Conclusion CEBPα mRNA is up-regulated at 0 h after PH, that is helpful for the expression of the G0 phase-related genes promoted by CEBPα and for the hepatocytes to be in G0 phase. On the contrary, the interaction of miR-144-3p and rno-LOC100365958_0001, rno-Usp3_0010 and rno-AC241873_0010 leads to CEBPα mRNA to bind with miR-144-3p, to CEBPα being formed difficultly, and to the expression of the G1 phase-related genes inbibited by CEBPα probablely, and to the hepatocytes being in G1 phase at 6 hours after PH.

Key words

Partial hepatectomy / Liver regeneration / Hepatocyte in G0 phase / Hepatocyte in G1 phase / CCAAT enhancer binding protein α / Competitive endogenous RNA comprehensive analysis / Biological high-throughput detection / Rat

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ZANG Xia-yan WANG Zi-hui LI Ya-fei GUO Jian-lin JIN WeiCHANG Cui-fang XU Cun-shuan. Expression and role of CCAAT enhancer binding protein α mRNA, microRNA-144-3p and three kinds of circular RNAs of hepatocytes during the rat liver regeneration initiation[J]. Acta Anatomica Sinica. 2021, 52(6): 901-908 https://doi.org/10.16098/j.issn.0529-1356.2021.06.009

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