Correlation between estrogen and progesterone fluctuation and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors in cortex and hippocampus during estrous cycle in mice

ZHENG Xiao-Min; ZHANG Ting-Yuan; ZHOU Tao; BU Chao-Zhi; YE Yang; ZHANG Yi-Xuan; REN Yong-Wei; JIANG Shi-Wen

doi:10.16098/j.issn.0529-1356.2021.06.001

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (6) : 839-844. DOI: 10.16098/j.issn.0529-1356.2021.06.001

Correlation between estrogen and progesterone fluctuation and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors in cortex and hippocampus during estrous cycle in mice

ZHENG Xiao-min^1,2,3* ZHANG Ting-yuan⁴ BU Chao-zhi¹ HUANG Lu¹ ZHOU Tao¹ YE Yang¹ ZHANG Yi-xuan¹ REN Yong-wei¹ JIANG Shi-wen^1*

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Abstract

Objective To explore the correlation of progesterone and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors (δGABA_ARs) in cerebral cortex and hippocampus， during different phase of estrus cycles in female mice. Methods Serum concentrations of estrogen and progesterone，of well-established ovarian cycles in female mice (4-6weeks, n=12) in estrus and di-estrus, had detected by ELISA kit. Immunohistochemistry was used to mark the difference expression of δGABA_ARs expression, in cerebral cortex and hippocampus during these two phases. Results The expression of δGABA_ARs was significant higher in di-estrus than estrus in hippocampus. The concentration of serum progesterone had co-inearity with integrated absorbance of δGABA_ARs in different phase of female estrus at the same brain region. Conclusion The physiological fluctuations of progesterone at different stages of estrus affect the expression of δGABA_ARs in the hippocampus and cortex and other specific brain regions. The differences in the expression of δGABA_ARs in hippocampal neurons will affect the excitability of brain neurons, which may further affect the behavioral characteristics of female animals.

Key words

γ-aminobutyric acid type A receptor / Estrus cycle / Progesterone / Cortex / Hippocampus / Immunohistochemistry / Mouse

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ZHENG Xiao-min ZHANG Ting-yuan BU Chao-zhi HUANG Lu ZHOU Tao YE Yang ZHANG Yi-xuan REN Yong-wei JIANG Shi-wen. Correlation between estrogen and progesterone fluctuation and expression of extrasynaptic δ-subunits containing γ-aminobutyric acid type A receptors in cortex and hippocampus during estrous cycle in mice[J]. Acta Anatomica Sinica. 2021, 52(6): 839-844 https://doi.org/10.16098/j.issn.0529-1356.2021.06.001

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