Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#

LIU Meng-Xin; SUN Chang-Ye; REN Ming-Fen; GUO Zhi-Kun; LI Duo

doi:10.16098/j.issn.0529-1356.2021.05.007

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (5) : 712-719. DOI: 10.16098/j.issn.0529-1356.2021.05.007

Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#

LIU Meng-xin¹ SUN Chang-ye¹ Li Duo¹ REN Ming-fen² GUO ZhI-kun^1*#br#

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Abstract

Objective To investigate the effect of fibroblast growth factor (FGF) on the proliferation and transdifferentiation of cardiac fibroblasts (CFs) into myofibroblasts (MFs). Methods Rat CFs were isolated and cultured, and then induced by FGF. CCK-8 was used to detect the cell activity and proliferation. Immunofluorescence and Western blotting were used to detect the expression of α smooth muscle actin (α-SMA) and collagen Ⅰ (ColⅠ). Results The expression and activation of α-SMA and ColⅠ increased with the increase of CFs culture generation. The number of CFs induced by FGF did not increased significantly; the expression of α-SMA in CFs induced by FGF1 and FGF2 decreased, and the number of activated MFs decreased. Conclusion FGF family has no effect on the proliferation of CFs, but FGF1 and FGF2 can inhibit the activation of CFs and reduce the differentiation into MFs.

Key words

Fibroblast growth factor / α-Smooth muscle actin / Myofibroblast / Cardiac fibroblast / Western blotting / Rat

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LIU Meng-xin SUN Chang-ye Li Duo REN Ming-fen GUO ZhI-kun. Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#[J]. Acta Anatomica Sinica. 2021, 52(5): 712-719 https://doi.org/10.16098/j.issn.0529-1356.2021.05.007

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