Dexmedetomidine alleviates formaldehyde induced pain by inhibiting astrocytes activation in spinal cord

ZENG Xue-Qing; YANG Bin; ZHOU Xin; YUAN Jing; XIE Zhi-Yan; LI Wei; WAN Wei; LIU Zhi-Wen; CAO Wen-Yu

doi:10.16098/j.issn.0529-1356.2021.05.002

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (5) : 681-685. DOI: 10.16098/j.issn.0529-1356.2021.05.002

Neurobiology

Dexmedetomidine alleviates formaldehyde induced pain by inhibiting astrocytes activation in spinal cord

ZENG Xue-qing¹ YANG Bin² ZHOU Xin³ YUAN Jing³ XIE Zhi-yan³ LI Wei³ WAN Wei³ LIU Zhi-wen^2* CAO Wen-yu^3*

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Abstract

Objective To investigate the effect of dexmedetomidine（DEX）on formaldehyde-induced acute inflammatory pain. Methods Thirty female ICR mice were randomly divided into three groups, including normal saline control (NS) group, formaldehyde（F）group and dexmedetomidine + formaldehyde (DEX+F) group. 0.1% formaldehyde solution was injected into subcutaneous tissue of mice right hind paw to establish acute inflammatory pain model. An hour before formalin injection, mice were intraperitoneal injected with DEX in DEX+F group, and mice were intraperitoneal injected with equal volume saline in NS group and F group. The expression of spinal cord glial fibrillary acidic protein (GFAP）, which is a astrocytes marker, was detected by immunohistochemistry and Western blotting. Results Spontaneous pain score showed that compared with the NS group, the F group had typical biphasic pain response; while compared with the F group, the DEX+F group showed a significant decrease in the first (P<0.001) and second phase pain (P<0.001). Immunohistochemical result showed that compared with the NS group, the F group had an increase number of GFAP positive cells in the posterior horn of the spinal cord (P<0.001). However, the DEX+F group showed a decrease number of GFAP positive cells in the posterior horn of spinal cord compared with the F group (P<0.001). Western blotting result showed that compared with the NS group, the F group had increased expression of GFAP in the spinal cord (P<0.05). Compared with the F group, the DEX+F group showed decreased GFAP expression in the spinal cord (P<0.05). Conclusion DEX may improve formaldehyde-induced acute inflammatory pain in mice by inhibiting the activation of astrocytes in the spinal cord.

Key words

Dexmedetomidine / Formaldehyde / Astrocyte / Acute inflammatory pain / Western blotting / Mouse

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ZENG Xue-qing YANG Bin ZHOU Xin YUAN Jing XIE Zhi-yan LI Wei WAN Wei LIU Zhi-wen CAO Wen-yu. Dexmedetomidine alleviates formaldehyde induced pain by inhibiting astrocytes activation in spinal cord[J]. Acta Anatomica Sinica. 2021, 52(5): 681-685 https://doi.org/10.16098/j.issn.0529-1356.2021.05.002

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