Determination of carbohydrate chains in two kinds of microvascular endothelial cells by lectin cytochemistry

YANG Si-Yu; YANG Zhong-Jin; ZHANG Zhi-Huan; MU Xiang; ZHANG Yong-Hong; ZHANG Tao

doi:10.16098/j.issn.0529-1356.2021.04.012

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (4) : 574-580. DOI: 10.16098/j.issn.0529-1356.2021.04.012

Cell and Molecules Biology

Determination of carbohydrate chains in two kinds of microvascular endothelial cells by lectin cytochemistry

YANG Si-yu¹ YANG Zhong-jin² ZHANG Zhi-huan¹ MU Xiang¹ ZHANG Yong-hong¹ ZHANG Tao^1*

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Abstract

Objective To determine the expression pattern of carbohydrate chains in two kinds of microvascular endothelial cells (MVECs). Methods Rat jejunal mucosal MVECs were thawed, and lung tissues were removed from specific pathogen free piglet of 3 days old to isolate and culture porcine pulmonary MVECs by collagerase digestion and differential attachment. By lectin cytochemistry, staining of 8 lectins including concanavalin A (Con A), phaseolus vulgaris erythroagglutinin (PHA-E), ricinus communis agglutinin Ⅰ (RCA-Ⅰ), lycopersicon esculentum lectin (LEL), sambucus nigra lectin (SNA), ulex europaeus agglutinin Ⅰ (UEA-Ⅰ), wheat germ agglutinin (WGA) and dolichos biflorus agglutinin (DBA) was detected in rat jejunal mucosal and porcine pulmonary MVECs. Results In rat jejunal mucosal MVECs, strong positve staining was present for Con A, WGA and LEL, medium one for PHA-E、SNA and RCA-Ⅰ, weak one for DBA, and negative staining for UEA-Ⅰ. In porcine pulmonary MVECs, strong positive staining was present for Con A and PHA-E, medium one in RCA-Ⅰ, weak one for LEL and SNA, and negative staining for UEA-Ⅰ, WGA and DBA. Conclusion The carbohydrate patterns in two kinds of MVECs display significant heterogeneity. Both rat jejunal mucosal and porcine pulmonary MVECs express mannose, galactose, 1, 3-N-acetylglucosamine and sialic acid at different levels. N-acetylglucosamine and N-acetylgalactosamine are detected in the former but not in the latter, and fucose do not in both MVECs.

Key words

Lung / Microvascular endothelial cell / Carbohydrate chain / Glycocalyx / Jejunal mucosa / Lectin / Cytochemical staining / Rat / Piglet

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YANG Si-yu YANG Zhong-jin ZHANG Zhi-huan MU Xiang ZHANG Yong-hong ZHANG Tao. Determination of carbohydrate chains in two kinds of microvascular endothelial cells by lectin cytochemistry[J]. Acta Anatomica Sinica. 2021, 52(4): 574-580 https://doi.org/10.16098/j.issn.0529-1356.2021.04.012

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