Role of Bcl-2 adenovirus/E1B 19kD interacting protein 3 in oligodendrocyte apoptosis after diffuse axonal injury

WANG Ting-Ting; MU Jiao; LI Mei-Yu; YU Hong; JIANG Cong-Zheng; ZHANG Xiao-Li; ZHANG Guo-Hui

doi:10.16098/j.issn.0529-1356.2021.04.003

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Acta Anatomica Sinica ›› 2021, Vol. 52 ›› Issue (4) : 512-519. DOI: 10.16098/j.issn.0529-1356.2021.04.003

Neurobiology

Role of Bcl-2 adenovirus/E1B 19kD interacting protein 3 in oligodendrocyte apoptosis after diffuse axonal injury

WANG Ting-ting¹ MU Jiao¹ LI Mei-yu¹ YU Hong¹ JIANG Cong-zheng¹ ZHANG Xiao-li² ZHANG Guo-hui^1*

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Abstract

Objective To investigate the role of Bcl-2 adenovirus/E1B 19kD interacting protein 3 (BNIP3) in oligodendrocyte apoptosis after diffuse axonal injury (DAI) in rats. Methods Seventy-seven male adult Sprague-Dawley rats were randomly divided into sham group (n=11), DAI group (n=33), and intervention group (n=33). DAI model was made referring to modified Marmarou method and the rats in intervention group received intracerebroventricular injection of BNIP3 inhibitor, necrostatin-1(Nec -1,30 g/L, 2 μl) immediately after injury. Tested the BNIP3 protein expression, oligodendrocyte apoptosis and myelin histopathology before and after the intervention of Nec-1. Results Compared with the sham group, DAI rats upregulated BNIP3 levels and had positive correlation with cell apoptosis in brainstem. Nec-1 significantly inhibited BNIP3 expression, then decreased the number of apoptotic oligodendrocytes, increased the average absorbance of luxol fast blue(LFB) staining and myelin basic protein (MBP) levels, and alleviated the myelin ultrastructure of DAI rats. Conclusion BNIP3 participate in the DAI-induced apoptosis of oligodendrocytes, and inhibition of BNIP3 can protect oligodendrocytes and myelin sheath from DAI injury.

Key words

Diffuse axonal injury / Bcl-2 adenovirus/E1B 19kD interacting protein 3 / Oligodendrocyte / Myelin / Immunohistochemistry / Western blotting / Rat

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WANG Ting-ting MU Jiao LI Mei-yu YU Hong JIANG Cong-zheng ZHANG Xiao-li ZHANG Guo-hui. Role of Bcl-2 adenovirus/E1B 19kD interacting protein 3 in oligodendrocyte apoptosis after diffuse axonal injury[J]. Acta Anatomica Sinica. 2021, 52(4): 512-519 https://doi.org/10.16098/j.issn.0529-1356.2021.04.003

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